Foecking M K, Hofstetter H
Gene. 1986;45(1):101-5. doi: 10.1016/0378-1119(86)90137-x.
The enhancer and promoter of the immediate early gene of human cytomegalovirus (hCMV) were tested as a transcriptional control element by fusion to the cat gene, followed by measurement of its expression from plasmid DNA in the extrachromosomal and integrated state, respectively. Comparison of the hCMV enhancer-promoter to two other viral elements was performed in six commonly used cell lines of different tissue and species origin. Irrespective of the cell line and of the state of the DNA, the hCMV enhancer-promoter was considerably stronger than both the SV40 promoter and the long terminal repeat of Rous sarcoma virus.
通过与氯霉素乙酰转移酶（cat）基因融合，将人巨细胞病毒（hCMV）即刻早期基因的增强子和启动子作为转录控制元件进行测试，随后分别测量其在染色体外和整合状态下从质粒DNA的表达。在六种不同组织和物种来源的常用细胞系中，将hCMV增强子-启动子与另外两种病毒元件进行了比较。无论细胞系和DNA状态如何，hCMV增强子-启动子都比SV40启动子和劳斯肉瘤病毒的长末端重复序列强得多。
