Fukasawa Y
Hokkaido Igaku Zasshi. 1986 Sep;61(5):682-9.
Human cytotoxic T cell (CTL) clones generated against a DQw3-positive EBV-transformed B cell line were analyzed in terms of immunological characteristics. The cytolytic activities of clones 1-18 and 3-34 were inhibited by anti-DQw3 monoclonal antibodies (HU-18 and HU-23), but they were not affected by anti-DR (HU-4 and HU-20) and anti-HLA-A, B, C (W6/32) monoclonal antibodies. In contrast to that the clone 1-18 cytolyzed all the human B cell lines typed as DQw3, the clone 3-34 reacted only with DR4/Dw4 and DR5-positive cells that were positive for HU-23 and typed as TA10. In addition, the clone 3-34 were cross-reactive to DQw2-typed cells. These results show that there are two epitopes that CTL recognize on the same DQw3 molecules; one is the public epitope among DQw3 antigens, and the other is the private epitope that HU-23 recognizes. This is the further evidence for CTL recognition of HLA-DQ antigens.
针对DQw3阳性的EB病毒转化B细胞系产生的人细胞毒性T细胞（CTL）克隆，从免疫学特征方面进行了分析。克隆1-18和3-34的细胞溶解活性受到抗DQw3单克隆抗体（HU-18和HU-23）的抑制，但不受抗DR（HU-4和HU-20）以及抗HLA-A、B、C（W6/32）单克隆抗体的影响。与克隆1-18能溶解所有分型为DQw3的人B细胞系不同，克隆3-34仅与DR4/Dw4和DR5阳性且对HU-23呈阳性反应并分型为TA10的细胞发生反应。此外，克隆3-34与DQw2分型的细胞存在交叉反应。这些结果表明，CTL在相同的DQw3分子上识别两个表位；一个是DQw3抗原中的公共表位，另一个是HU-23识别的私有表位。这是CTL识别HLA-DQ抗原的进一步证据。
