地尔硫䓬促进再生轴突生长。
Diltiazem Promotes Regenerative Axon Growth.
机构信息
F.M. Kirby Neurobiology Center, Boston Children's Hospital and Harvard Medical School, Boston, MA, 02115, USA.
Cellular Neuroscience, Neurodegeneration and Repair Program, Departments of Neurology and Neuroscience, Yale University School of Medicine, New Haven, CT, 06520, USA.
出版信息
Mol Neurobiol. 2019 Jun;56(6):3948-3957. doi: 10.1007/s12035-018-1349-5. Epub 2018 Sep 19.
Abstract
Axotomy results in permanent loss of function after brain and spinal cord injuries due to the minimal regenerative propensity of the adult central nervous system (CNS). To identify pharmacological enhancers of axon regeneration, 960 compounds were screened for cortical neuron axonal regrowth using an in vitro cortical scrape assay. Diltiazem, verapamil, and bromopride were discovered to facilitate axon regeneration in rat cortical cultures, in the presence of chondroitin sulfate proteoglycans (CSPGs). Diltiazem, an L-type calcium channel blocker (L-CCB), also promotes axon outgrowth in adult primary mouse dorsal root ganglion (DRG) and induced human sensory (iSensory) neurons.
摘要
轴突切断会导致脑和脊髓损伤后的永久性功能丧失，这是由于成人中枢神经系统（CNS）的再生能力极弱所致。为了鉴定促进轴突再生的药物，使用体外皮质刮擦测定法筛选了 960 种化合物以促进皮质神经元轴突再生。发现地尔硫卓、维拉帕米和溴必利在软骨素硫酸盐蛋白聚糖（CSPG）存在的情况下有利于大鼠皮质培养物中的轴突再生。地尔硫卓是一种 L 型钙通道阻滞剂（L-CCB），也可促进成年原发性小鼠背根神经节（DRG）中的轴突生长，并诱导人感觉神经元（iSensory 神经元）。
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本文引用的文献
1
The Extracellular Environment of the CNS: Influence on Plasticity, Sprouting, and Axonal Regeneration after Spinal Cord Injury.
Neural Plast. 2018 Apr 18;2018:2952386. doi: 10.1155/2018/2952386. eCollection 2018.
2
Effects of Long- and Intermediate-Acting Dihydropyridine Calcium Channel Blockers in Hypertension: A Systematic Review and Meta-Analysis of 18 Prospective, Randomized, Actively Controlled Trials.
J Cardiovasc Pharmacol Ther. 2018 Sep;23(5):433-445. doi: 10.1177/1074248418771341. Epub 2018 May 8.
3
Investigational drugs for vasospasm after subarachnoid hemorrhage.
Expert Opin Investig Drugs. 2018 Apr;27(4):313-324. doi: 10.1080/13543784.2018.1460353. Epub 2018 Apr 6.
4
Neuroprotective effect of lercanidipine in middle cerebral artery occlusion model of stroke in rats.
Exp Neurol. 2017 Feb;288:25-37. doi: 10.1016/j.expneurol.2016.10.014. Epub 2016 Oct 26.
5
Attenuation of Axonal Degeneration by Calcium Channel Inhibitors Improves Retinal Ganglion Cell Survival and Regeneration After Optic Nerve Crush.
Mol Neurobiol. 2017 Jan;54(1):72-86. doi: 10.1007/s12035-015-9676-2. Epub 2016 Jan 5.
6
Robust Axonal Regeneration Occurs in the Injured CAST/Ei Mouse CNS.
Neuron. 2015 Jun 3;86(5):1215-27. doi: 10.1016/j.neuron.2015.05.005. Epub 2015 May 21.
7
Nimodipine enhances neurite outgrowth in dopaminergic brain slice co-cultures.
Int J Dev Neurosci. 2015 Feb;40:1-11. doi: 10.1016/j.ijdevneu.2014.10.005. Epub 2014 Nov 4.
8
Role of CSPG receptor LAR phosphatase in restricting axon regeneration after CNS injury.
Neurobiol Dis. 2015 Jan;73:36-48. doi: 10.1016/j.nbd.2014.08.030. Epub 2014 Sep 8.
9
Effect and reporting bias of RhoA/ROCK-blockade intervention on locomotor recovery after spinal cord injury: a systematic review and meta-analysis.
JAMA Neurol. 2014 Jan;71(1):91-9. doi: 10.1001/jamaneurol.2013.4684.
10
Voltage-gated calcium channel antagonists and traumatic brain injury.
Pharmaceuticals (Basel). 2013 Jun 26;6(7):788-812. doi: 10.3390/ph6070788.
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