The Gene From Regulate the Resistance Against Cereal Cyst Nematode by Altering the Downstream Secondary Metabolite Contents Rather Than Auxin Synthesis.

Cereal cyst nematode (CCN, ) is a most important pathogen of wheat and causes tremendous yield loss annually over the world. Since the lack of resistance materials among wheat cultivars, identification and characterization of the resistance-related genes from the relatives of wheat is a necessary and efficient way. As a close relative of wheat with high resistance against CCN, is believed to be a valuable source for wheat breeding against this devastating disease. However so far, very few resistance-associated genes have been characterized from this species. In this study, we present that the genes from ( and ) were both induced by CCN juveniles at the early stage of resistance response (30 h post-inoculation), with more sensitive to CCN infection than . Silencing of led to compromised immunity to CCN with more CCN intrusion into roots; while overexpression in dramatically enhanced the resistance of plants by reducing the knots formed on roots. Metabolism analysis showed that the contents of secondary metabolites with activity of resistance to varied pathogens correlated with the expression level of in both and the transgenic tobacco plants. In addition, the content of IAA was not affected by either silencing or overexpressing of . Hence, our research provided a valuable target that mediates resistance to CCN and root knot nematode (RKN, ) without influencing the auxin biosynthesis.