Coriton Olivier, Barloy Dominique, Huteau Virginie, Lemoine Jocelyne, Tanguy Anne-Marie, Jahier Joseph
UMR 118 APBV-INRA - Agrocampus Ouest-Universite de Rennes 1, BP 35327, F-35653 Le Rheu, France.
Genome. 2009 Apr;52(4):338-46. doi: 10.1139/g09-011.
The allotetraploid species Aegilops variabilis Eig (2n = 28, UUSvSv) belongs to the tribe Triticeae and is closely related to wheat. One accession, Ae. variabilis No. 1, was found to be resistant to the cereal cyst nematode (CCN) and the root-knot nematode (RKN). As the genetic variability for resistance to those two pests is limited within wheat, this accession was crossed to bread wheat. Previous work enabled the development of two addition lines and two translocation lines carrying resistance. Here, we demonstrate, using genomic in situ hybridization, that there is no U-Sv interchange in the parental accession of Ae. variabilis. However, there are multiple rearrangements in the Sv chromosomes. The Ae. variabilis chromosome carrying the CreX gene for resistance to CCN combined segments with homoeology to wheat groups 1, 2, 4, and 6. The CreX gene belongs to the group 1 part and it was likely to have been introduced into chromosome 1BL at a similar location as the previously found QTL QCre.srd-1B for CCN resistance. The second Ae. variabilis chromosome carrying CreY and Rkn2 combined segments with homoeology to wheat groups 2, 4, and 7 on its short arm and group 3 on its long arm. It was designated as 3Sv. The two genes for resistance are carried by its long arm and have been transferred to wheat chromosome 3BL through homoeologous and genetically balanced recombination. Different SSR markers present in the introgressed segments could be used in marker-assisted selection.
异源四倍体物种易变山羊草（Aegilops variabilis Eig，2n = 28，UUSvSv）属于小麦族，与小麦亲缘关系密切。发现一份易变山羊草材料，编号为1号，对禾谷孢囊线虫（CCN）和根结线虫（RKN）具有抗性。由于小麦中对这两种害虫的抗性遗传变异有限，该材料与面包小麦进行了杂交。先前的工作培育出了两个附加系和两个携带抗性的易位系。在此，我们利用基因组原位杂交证明，在易变山羊草的亲本材料中不存在U-Sv互换。然而，Sv染色体存在多个重排。携带抗CCN的CreX基因的易变山羊草染色体与小麦第1、2、4和6组具有同源性的片段结合。CreX基因属于第1组部分，可能已在与先前发现的抗CCN的QTL QCre.srd-1B相似的位置引入到1BL染色体中。第二条携带CreY和Rkn2的易变山羊草染色体短臂上与小麦第2、4和7组具有同源性，长臂上与第3组具有同源性。它被命名为3Sv。两个抗性基因由其长臂携带，并通过同源和遗传平衡重组转移到小麦3BL染色体上。渗入片段中存在的不同SSR标记可用于标记辅助选择。
