Koshida M, Okamura H, Mori T, Takenaka A
Nihon Sanka Fujinka Gakkai Zasshi. 1986 Oct;38(10):1741-6.
Various doses of rotenone, an inhibitor of respiratory chain, were administered to mature female rabbits concomitantly with an ovulatory dose of hCG. The effect of rotenone on ovulation was studied by counting the ovulated stigma under a dissecting microscope. Histochemical studies on the activities of 3 beta-hydroxysteroid dehydrogenase (3 beta-HSD) and cytochrome oxidase (CYO) in follicles at various intervals after hCG-rotenone injections were also performed to investigate the role of mitochondrial oxidation in the ovulatory process. Rotenone inhibited the hCG-induced ovulation in a dose respondent manner and reduced the sudden hCG-induced increase in the histochemical activities of 3 beta-HSD and CYO of granulosa cells. It is suggested that the activation of mitochondrial oxidation in the ovulating follicle is mandatory for ovulation and the induction of steroidogenic enzymes in granulosa cells.
将呼吸链抑制剂鱼藤酮的不同剂量与排卵剂量的人绒毛膜促性腺激素（hCG）同时给予成年雌性兔子。通过在解剖显微镜下计数排卵点来研究鱼藤酮对排卵的影响。还对注射hCG - 鱼藤酮后不同时间间隔的卵泡中3β - 羟基类固醇脱氢酶（3β - HSD）和细胞色素氧化酶（CYO）的活性进行了组织化学研究，以探讨线粒体氧化在排卵过程中的作用。鱼藤酮以剂量反应方式抑制hCG诱导的排卵，并减少hCG诱导的颗粒细胞3β - HSD和CYO组织化学活性的突然增加。提示排卵卵泡中线粒体氧化的激活对排卵和颗粒细胞中类固醇生成酶的诱导是必不可少的。
