Cohen R B, Cohen S
J Exp Pathol. 1985 Fall;2(3):171-6.
A histochemical technique, developed for the localization of DHO-D activity in tissue, has been utilized to study the distribution of this activity in rabbit spleen. This enzyme is involved in the de novo synthesis of pyrimidines of nucleic acids via orotic acid as an intermediate. The enzyme was found to be most active at the periphery of follicles in the resting spleen. Following antigenic challenge, enzyme activity became prominent for several days in macrophages within germinal centers, but not in the intensely pyroninophilic cells which are the dominant cell type of these structures. Antigenicity of the injected compound appeared to be necessary for the production of this effect. The pattern of localization of DHO-D is similar to the pattern of distribution of antigen within splenic tissue.
一种用于在组织中定位二氢乳清酸脱氢酶（DHO-D）活性的组织化学技术，已被用于研究该活性在兔脾脏中的分布。这种酶通过乳清酸作为中间体参与核酸嘧啶的从头合成。发现该酶在静息脾脏的滤泡周边最为活跃。抗原刺激后，生发中心内的巨噬细胞中酶活性在数天内变得显著，但在这些结构中占主导细胞类型的强嗜派洛宁细胞中则不显著。注射化合物的抗原性似乎是产生这种效应所必需的。DHO-D的定位模式与脾组织内抗原的分布模式相似。
