Pan Ying, Yan Bo, Wang Chuanbo, Wang Hongjie, Wang Qi, Liu Xiaoyan
Anhui Medical College, Hefei 230601, China. *Corresponding author, E-mail:
Anhui Medical College, Hefei 230601, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2018 Jun;34(6):511-516.
Objective To investigate the effect of interleukin 27 (IL-27) on hepatitis B virus (HBV) replication and antigen secretion in HepG2.2.15 cells and related mechanisms. Methods HepG2.2.15 cells were treated with IL-27 at various doses. The levels of HBsAg and HBeAg in the supernatant of the cell cultures were measured by ELISA, and the levels of HBV DNA was detected by real-time quantitative PCR. The expression and localization of HBV core antigen (HBcAg) in HepG2.2.15 cells were observed by immunocytochemical staining. Moreover, the expression of STAT1, phosphorylated STAT1 (p-STAT1) and myxovirus-resistance protein A (MxA) were analyzed by Western blotting. Results After the treatment with different doses of IL-27, the levels of HBV-DNA, HBsAg, HBeAg and intracellular HBcAg in HepG2.2.15 cells gradually decreased. With the increase of IL-27 dose, the expression level of p-STAT1 significantly increased. There was no significant change in STAT1 expression. Further studies also showed that IL-27 induced MxA expression in a dose- and time-dependent manner in HepG2.2.15 cells. Conclusion IL-27 could induces MxA expression by activating STAT signaling pathway to exert anti-HBV activity.
目的 探讨白细胞介素27（IL-27）对HepG2.2.15细胞中乙型肝炎病毒（HBV）复制及抗原分泌的影响及其相关机制。方法 用不同剂量的IL-27处理HepG2.2.15细胞。采用酶联免疫吸附测定法（ELISA）检测细胞培养上清液中HBsAg和HBeAg水平，采用实时定量聚合酶链反应（real-time quantitative PCR）检测HBV DNA水平。通过免疫细胞化学染色观察HepG2.2.15细胞中HBV核心抗原（HBcAg）的表达及定位。此外，采用蛋白质免疫印迹法（Western blotting）分析信号转导和转录激活因子1（STAT1）、磷酸化信号转导和转录激活因子1（p-STAT1）及抗黏液病毒蛋白A（MxA）的表达。结果 用不同剂量的IL-27处理后，HepG2.2.1细胞中HBV-DNA、HBsAg、HBeAg及细胞内HBcAg水平逐渐降低。随着IL-27剂量增加，p-STAT1表达水平显著升高，STAT1表达无明显变化。进一步研究还显示，IL-27在HepG2.2.15细胞中呈剂量和时间依赖性诱导MxA表达。结论IL-27可通过激活信号转导和转录激活因子（STAT）信号通路诱导MxA表达，从而发挥抗HBV活性。
