Streinzer W, Krammer E B
J Neurosci Methods. 1986 Sep;17(4):297-301. doi: 10.1016/0165-0270(86)90130-5.
A combination of two procedures, embedding of specimens and horseradish peroxidase (HRP) histochemistry on mounted serial sections, is proposed for three-dimensional reconstruction of neuronal projections. To clarify the intrinsic organization of the peripheral nervous system, this simple method is more adequate than previously used techniques.
提出了一种将标本包埋和在连续切片上进行辣根过氧化物酶(HRP)组织化学这两种方法相结合的技术,用于神经元投射的三维重建。为了阐明周围神经系统的内在组织结构,这种简单方法比以前使用的技术更为适用。