Faculty of Medical Biotechnology, Department of Stem Cells and Regenerative Medicine, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran.
Cell Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Cell Biol Int. 2018 Dec;42(12):1688-1694. doi: 10.1002/cbin.11056. Epub 2018 Oct 2.
Neural differentiation involves drastic morphological alterations, essentially performed by a cell-homeostasis maintaining process known as autophagy. Here, we used the cocktail of choroid plexus epithelial cell-conditioned medium (CPEC-CM) and 15% knockout serum (KS) to induce human adipose-derived mesenchymal stem cells (hASCs) into tyrosine hydroxylase (TH)-positive neuron like cells. We showed that upon this induction, autophagy pathway was transcriptionally triggered. The expression levels of autophagy markers mTOR, BECN1, and MAP1LC3 were evidently changed throughout the dopaminergic (DAergic) differentiation of hASCs, highlighting the critical role of autophagy in this process at the level of transcription.
神经分化涉及剧烈的形态改变，主要通过一种称为自噬的细胞稳态维持过程来完成。在这里，我们使用脉络丛上皮细胞条件培养基 (CPEC-CM) 和 15% 无血清 (KS) 混合物诱导人脂肪间充质干细胞 (hASCs) 分化为酪氨酸羟化酶 (TH)阳性神经元样细胞。我们发现，在这种诱导下，自噬途径被转录触发。自噬标志物 mTOR、BECN1 和 MAP1LC3 的表达水平在 hASCs 的多巴胺能 (DAergic) 分化过程中明显变化，突出了自噬在转录水平上在此过程中的关键作用。
