Pathirana H N K S, Shin G W, Wimalasena S H M P, Hossain S, De Silva B C J, Dahanayake P S, Heo G-J
Laboratory of Aquatic Animal Medicine, Veterinary Medical Center and College of Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk, Korea.
Bio-Safety Research Institute and College of Veterinary Medicine, Chonbuk National University, Jeonju, Korea.
Lett Appl Microbiol. 2018 Dec;67(6):620-627. doi: 10.1111/lam.13077. Epub 2018 Nov 12.
Proteeae is a tribe which consists of three genera: Proteus, Providencia and Morganella. The objective of this study was to determine antimicrobial resistance profile, virulence genotype and class 1 and 2 integrons in Proteeae isolated from pet turtles and to determine the impact of antibiotic resistance on virulence and antimicrobial resistance genes. Integron-positive isolates were used to detect their gene cassette array. Sixty four Proteeae were isolated and all were resistant to macrolides (100%). Among 64 isolates 56, 52, 36 and 25 were resistant to nitrofurans, β-lactams, tetracycline and aminoglycoside respectively. Sixteen (25%) isolates were positive for intI1 while 14 (21·87%) were positive for integrase 2 (intI2). Eleven (17·18%) isolates were positive for class 1 variable region while 7 (10·93%) were positive for class 2 variable region. IMP27, a novel metallo β-lactamase gene was found in Providencia isolates. Proteus sp. were positive for every tested virulence genes and UreC gene was detected in 48·44% followed by zapA (17·19%), mrpA (17·19%) and hlyA (14·06%) genes. In this study, integron associated-antibiotic resistance genes have been identified in Proteeae isolates in a considerable range representing clear threats to public health. SIGNIFICANCE AND IMPACT OF THE STUDY: In this study, multidrug-resistant Proteeae isolates had several antibiotic resistance genes. Integrons are important contributors to the development and dissemination of antibiotic resistance. We could detect both class 1 and 2 integrons and several gene cassette arrays in class 1 integron. The gene cassette arrays of the Class 2 integrons contained IMP27-dfrA1-aadA1-catB2-ybeA-ybgA in two isolates. To the best of our knowledge this is the first study to report detection of IMP27 in Providencia rettgeri isolates. All results indicate that healthy pet turtles act as potential reservoirs for Proteeae species with zoonotic potential.
变形杆菌族是一个由变形杆菌属、普罗威登斯菌属和摩根菌属三个属组成的菌族。本研究的目的是确定从宠物龟分离出的变形杆菌族的抗菌药物耐药谱、毒力基因型以及1类和2类整合子，并确定抗生素耐药性对毒力和抗菌耐药基因的影响。对整合子阳性分离株检测其基因盒阵列。共分离出64株变形杆菌族菌株，所有菌株均对大环内酯类耐药（100%）。64株分离株中，分别有56株、52株、36株和25株对硝基呋喃类、β-内酰胺类、四环素类和氨基糖苷类耐药。16株（25%）分离株intI1呈阳性，14株（21.87%）整合酶2（intI2）呈阳性。11株（17.18%）分离株1类可变区呈阳性，7株（10.93%）2类可变区呈阳性。在普罗威登斯菌属分离株中发现了新型金属β-内酰胺酶基因IMP27。变形杆菌属的所有测试毒力基因均呈阳性，48.44%的菌株检测到脲酶C基因，其次是zapA（17.19%）、mrpA（17.19%）和hlyA（14.06%）基因。在本研究中，已在相当比例的变形杆菌族分离株中鉴定出与整合子相关的抗生素耐药基因，这对公共卫生构成了明显威胁。研究的意义和影响：在本研究中，多重耐药的变形杆菌族分离株含有多个抗生素耐药基因。整合子是抗生素耐药性产生和传播的重要因素。我们能够检测到1类和2类整合子以及1类整合子中的几个基因盒阵列。2类整合子的基因盒阵列在两株分离株中包含IMP27-dfrA1-aadA1-catB2-ybeA-ybgA。据我们所知，这是首次报道在雷氏普罗威登斯菌分离株中检测到IMP27。所有结果表明，健康的宠物龟是具有人畜共患病潜力的变形杆菌族物种的潜在储存宿主。
