Marlor R L, Parkhurst S M, Corces V G
Mol Cell Biol. 1986 Apr;6(4):1129-34. doi: 10.1128/mcb.6.4.1129-1134.1986.
We determined the complete nucleotide sequence of the gypsy element present at the forked locus of Drosophila melanogaster in the f1 allele. The gypsy element shares more homology with vertebrate retroviruses than with the copia element of D. melanogaster or the Ty element of Saccharomyces cerevisiae, both in overall organization and at the DNA sequence level. This transposable element is 7,469 base pairs long and encodes three putative protein products. The long terminal repeats are 482 nucleotides long and contain transcription initiation and termination signals; sequences homologous to the polypurine tract and tRNA primer binding site of retroviruses are located adjacent to the long terminal repeats. The central region of the element contains three different open reading frames. The second one encodes a putative protein which shows extensive amino acid homology to retroviral proteins, including gag-specific protease, reverse transcriptase, and DNA endonuclease.
我们确定了位于果蝇f1等位基因中叉状位点的gypsy元件的完整核苷酸序列。gypsy元件在整体结构和DNA序列水平上，与脊椎动物逆转录病毒的同源性比与黑腹果蝇的copia元件或酿酒酵母的Ty元件更高。这个转座元件长7469个碱基对，编码三种假定的蛋白质产物。长末端重复序列长482个核苷酸，包含转录起始和终止信号；与逆转录病毒的多嘌呤序列和tRNA引物结合位点同源的序列位于长末端重复序列附近。该元件的中心区域包含三个不同的开放阅读框。第二个开放阅读框编码一种假定的蛋白质，它与逆转录病毒蛋白具有广泛的氨基酸同源性，包括gag特异性蛋白酶、逆转录酶和DNA内切酶。
