School of Chemical Science and Engineering and Shanghai Key Laboratory of Chemical Assessment and Sustainability, Tongji University, Shanghai, P. R. China.
J Sep Sci. 2018 Nov;41(22):4194-4202. doi: 10.1002/jssc.201800689. Epub 2018 Oct 10.
In this study, a facile synthesis of the titanium(IV) ion-immobilized poly-glycidyl methacrylate microparticles functionalized with polyethylenimine and adenosine triphosphate was developed for efficient enrichment of intact phosphoproteins. The titanium(IV) ion-immobilized microparticles had higher saturated adsorption capacity for phosphoproteins (1217.6 mg/g for β-casein) than nonphosphoproteins (97.1 mg/g for bovine serum albumin) and the average particle diameter was about 1.4 μm with good dispersibility. In application, as demonstrated by SDS-PAGE, titanium(IV) ion-immobilized microparticles exhibited good performance in enriching intact phosphoproteins from standard protein mixtures of β-casein and bovine serum albumin with high specificity and selectivity. In addition, titanium(IV) ion-immobilized microparticles were also successfully applied in intact phosphoprotein enrichment from complex biological samples including nonfat milk, chicken egg white, and mouse heart tissue extract.
在这项研究中，开发了一种简便的钛(IV)离子固定化聚甲基丙烯酸缩水甘油酯微球的方法，该微球通过聚亚乙基亚胺和三磷酸腺苷进行功能化，可用于高效富集完整的磷酸化蛋白。钛(IV)离子固定化微球对磷酸化蛋白（β-乳球蛋白为 1217.6mg/g）的饱和吸附容量高于非磷酸化蛋白（牛血清白蛋白为 97.1mg/g），平均粒径约为 1.4μm，具有良好的分散性。在应用中，如 SDS-PAGE 所示，钛(IV)离子固定化微球在从β-乳球蛋白和牛血清白蛋白的标准蛋白质混合物中特异性和选择性地富集完整的磷酸化蛋白方面表现出良好的性能。此外，钛(IV)离子固定化微球还成功地应用于从复杂的生物样品（包括脱脂牛奶、鸡蛋白和鼠心脏组织提取物）中富集完整的磷酸化蛋白。
