Department of Clinical Sciences, North Carolina State University College of Veterinary Medicine, Raleigh, North Carolina.
HLA. 2018 Dec;92(6):403-407. doi: 10.1111/tan.13398. Epub 2018 Nov 7.
Development of effective immunotherapy for chemoresistant malignancies can be advanced by studies in spontaneous cancer models, such as the dog. A crucial first step, T-cell epitope discovery, can be assisted by determination of binding motifs of common dog leukocyte antigen (DLA) class Ia allotypes. Boxers are popular, inbred dogs with increased risks of relevant target cancers and restricted MHC diversity. We sought to identify the motif of DLA-88*034:01, a breed-dominant allotype, to assist peptide prediction from tumor antigens. Mass spectrometry of eluted peptides showed a preference for nonamers with conserved amino acid preferences: basic at position (P)1; hydrophobic at P2; acidic at P4; histidine at P6; and phenylalanine at P9. This data should expedite finding epitopes restricted by this DLA-88 allotype.
通过对自发性癌症模型（如狗）的研究，可以推进针对耐药性恶性肿瘤的有效免疫疗法的发展。关键的第一步是 T 细胞表位的发现，这可以通过确定常见犬白细胞抗原（DLA）I 类同种异型的结合基序来辅助。拳击犬是一种受欢迎的近交犬种，其相关靶癌症风险增加，且 MHC 多样性受到限制。我们试图确定 DLA-88*034:01 的基序，这是一种品种优势同种异型，以协助从肿瘤抗原中预测肽。洗脱肽的质谱分析显示对具有保守氨基酸偏好的非九肽有偏好：在位置 P1 为碱性；P2 为疏水性；P4 为酸性；P6 为组氨酸；P9 为苯丙氨酸。这些数据应该可以加快找到受这种 DLA-88 同种异型限制的表位。
