Shandong Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan 250100, People's Republic of China.
Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, 61 Daizong Street, Taian City, Shandong Province 271018, China.
Poult Sci. 2019 Feb 1;98(2):573-580. doi: 10.3382/ps/pey425.
In this study, we determined and analyzed the complete nucleotide sequence of the genome of a fowl adenovirus isolate SD1356 in China and examined its pathogenicity in specific pathogen-free chick embryos and newly hatched chicks. The full genome of SD1356 was 44,454 nucleotides in length with 58.1% G + C content. Sequence alignment and phylogenetic analysis revealed that strain SD1356 was clustered together belonging to serotype 8b of fowl adenoviruses E species (FAdV-8b). No regions homologous to early regions E1, E3, and E4 of mastadenoviruses were recognized, and being very similar to the typical organization of FAdV-E genomes. All infected embryos died 4-6 d post-inoculation with visible lesions, such as hyperemic, stunting, and clubbed down, etc. Additionally, adenovirus was found in tissues or cloacal swabs of all infected birds and most of the contact uninfected controls, despite lack of clinical signs and pathological changes. Together, our study describes the genomic characteristics of an FAdV-8b strain isolated in China. The reported FAdV-8b strain SD1356 is fetal to chick embryos and possesses horizontal transmission capacity in chickens.
在这项研究中，我们测定并分析了中国分离株 SD1356 禽腺病毒的全基因组序列，并研究了其在 SPF 鸡胚和刚孵出的雏鸡中的致病性。SD1356 的全基因组长度为 44454 个核苷酸，GC 含量为 58.1%。序列比对和系统进化分析表明，该分离株 SD1356 属于禽腺病毒 E 亚群 8b 血清型（FAdV-8b）。未发现与哺乳动物腺病毒早期区 E1、E3 和 E4 同源的区域，与典型的 FAdV-E 基因组组织非常相似。所有感染的胚胎在接种后 4-6 天死亡，出现肉眼可见的病变，如充血、发育迟缓、足部肿胀等。此外，在所有感染鸟类的组织或泄殖腔拭子中以及大多数接触未感染对照的鸟类中都发现了腺病毒，尽管没有临床症状和病理变化。总之，本研究描述了在中国分离的一株 FAdV-8b 株的基因组特征。所报道的 FAdV-8b 株 SD1356 对鸡胚是致死性的，并且在鸡中有水平传播的能力。
