Kochman R L, Sepulveda C K
Neurobiol Aging. 1986 Sep-Oct;7(5):363-5. doi: 10.1016/0197-4580(86)90163-6.
The effects of GABA on benzodiazepine receptor binding in cerebral cortical, hippocampal, and cerebellar membranes from 2-3 months old and 28-32 months old rats were studied. GABA modulation of agonist, antagonist, and inverse agonist binding to the receptor was examined using the displacement of 3H-Ro15-1788 by diazepam, Ro15-1788, and beta-carboline-3-carboxylate methyl ester, respectively, in the absence and presence of 100 microM GABA and 150 mM sodium chloride. GABA modulation was alike in old and young rats, with respect to the particular ligand and brain region. The results support the hypothesis that, in the brain regions studied, the allosteric modulation of benzodiazepine receptor binding by GABA remains intact as a function of aging.
研究了γ-氨基丁酸（GABA）对2至3月龄和28至32月龄大鼠大脑皮质、海马和小脑膜中苯二氮䓬受体结合的影响。分别在不存在和存在100微摩尔GABA和150毫摩尔氯化钠的情况下，使用地西泮、Ro15 - 1788和β-咔啉-3-羧酸甲酯取代3H-Ro15 - 1788，检测了GABA对激动剂、拮抗剂和反向激动剂与该受体结合的调节作用。就特定配体和脑区而言，老年和幼年大鼠的GABA调节作用相似。结果支持这样的假说：在所研究的脑区中，随着衰老，GABA对苯二氮䓬受体结合的变构调节作用保持完整。
