Jacobson W, Wilkinson M
Neuroendocrinology. 1986;44(1):132-5. doi: 10.1159/000124634.
We report the existence of a diurnal variation in the binding of the opiate antagonist [3H]-naloxone to slices of the mediobasal hypothalamus from prepubertal female rats. The binding is highest in the early morning and reaches a nadir in the late afternoon. Opiate binding in cortical slices from such animals is constant over the course of the day. Changes in receptor density, and not in receptor affinity, account for the diurnal variation in the amount of ligand bound. These diurnal variations in receptor numbers are associated with changes in the ability of naloxone to release LH and may be crucial in the transition from the juvenile state to one of competent reproductive functioning.
我们报告了阿片拮抗剂[3H] - 纳洛酮与青春期前雌性大鼠下丘脑中间基底部切片结合存在昼夜变化。结合在清晨最高，在傍晚降至最低点。此类动物皮质切片中的阿片结合在一天中保持恒定。配体结合量的昼夜变化是由受体密度变化而非受体亲和力变化引起的。受体数量的这些昼夜变化与纳洛酮释放促黄体生成素（LH）能力的变化相关，并且可能在从幼年状态向具备生殖功能状态的转变中起关键作用。
