将[F]氟化物自动浓缩至微升体积。
Automated concentration of [F]fluoride into microliter volumes.
作者信息
Chao Philip H, Lazari Mark, Hanet Sebastian, Narayanam Maruthi Kumar, Murphy Jennifer M, van Dam R Michael
机构信息
Department of Bioengineering, Henry Samueli School of Engineering, UCLA, Los Angeles, CA 90095, USA; Crump Institute for Molecular Imaging, David Geffen School of Medicine, UCLA, Los Angeles, CA 90095, USA; Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, UCLA, Los Angeles, CA 90095, USA.
Crump Institute for Molecular Imaging, David Geffen School of Medicine, UCLA, Los Angeles, CA 90095, USA; Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, UCLA, Los Angeles, CA 90095, USA.
出版信息
Appl Radiat Isot. 2018 Nov;141:138-148. doi: 10.1016/j.apradiso.2018.06.017. Epub 2018 Jun 23.
Concentration of [F]fluoride has been mentioned in literature, however, reports have lacked details about system designs, operation, and performance. Here, we describe in detail a compact, fast, fully-automated concentration system based on a micro-sized strong anion exchange cartridge. The concentration of radionuclides enables scaled-up microfluidic synthesis. Our system can also be used to provide highly concentrated [F]fluoride with minimal water content. We demonstrate how the concentrator can produce varying concentrations of [F]fluoride for the macroscale synthesis of N-boc-5-[F]fluoroindole without an azeotropic drying process, while enabling high starting radioactivity. By appropriate choice of solid-phase resin, flow conditions, and eluent solution, we believe this approach can be extended beyond [F]fluoride to other radionuclides.
文献中已提及[F]氟化物的浓缩，但关于系统设计、操作和性能的报告缺乏细节。在此，我们详细描述了一种基于微型强阴离子交换柱的紧凑、快速、全自动浓缩系统。放射性核素的浓缩可实现微流控合成的放大。我们的系统还可用于提供含水量极低的高浓度[F]氟化物。我们展示了该浓缩器如何在无需共沸干燥过程的情况下，为N-叔丁氧羰基-5-[F]氟吲哚的宏观合成产生不同浓度的[F]氟化物，同时实现高起始放射性。通过适当选择固相树脂、流动条件和洗脱液，我们相信这种方法可扩展到[F]氟化物以外的其他放射性核素。
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