Eiras Matheus Credendio, Miranda-Furtado Cristiana Libardi, Fernandes Artur, Ramos Ester Silveira
Department of Genetics, Ribeirao Preto Medical School - University of São Paulo (USP), São Paulo, São Paulo, Brazil.
Department of Genetics, Ribeirao Preto Medical School - University of São Paulo (USP), São Paulo, São Paulo, Brazil; Drug Research and Development Center, Federal University of Ceara, Fortaleza, Ceara, Brazil.
Theriogenology. 2018 Dec;122:84-87. doi: 10.1016/j.theriogenology.2018.09.015. Epub 2018 Sep 15.
Birds have a ZZ and ZW sex chromosome system (male and female, respectively). On the short arm of the Z chromosome of Gallus gallus domesticus there is a repetitive region called MHM region, which is absent on the W chromosome, that causes a natural copy number difference of the MHM region between the sexes, making possible the development of a quantitative PCR (qPCR) sexing assay, based on the Z chromosome double dose. Twenty-seven samples of tissues from eight adult Gallus gallus domesticus (four males and four females) were used to establish the parameters of the MHM copy-number sexing assay. We blinded sexed 20 chicks using 140 samples from different tissues (heart, brain, gonad, kidney, lung, muscle, and blood). The success rate of the assay was 100% (140/140). It required small amounts of DNA (0.39 ng), opening the perspective to the use of the assay in studies in which the amount of available DNA is limited.
鸟类具有ZZ和ZW性染色体系统（分别为雄性和雌性）。在家鸡的Z染色体短臂上有一个称为MHM区域的重复区域，W染色体上没有该区域，这导致两性之间MHM区域的天然拷贝数差异，使得基于Z染色体双倍剂量的定量PCR（qPCR）性别鉴定分析得以发展。使用来自8只成年家鸡（4只雄性和4只雌性）的27个组织样本建立MHM拷贝数性别鉴定分析的参数。我们使用来自不同组织（心脏、大脑、性腺、肾脏、肺、肌肉和血液）的140个样本对20只雏鸡进行了盲法性别鉴定。该分析的成功率为100%（140/140）。它需要少量的DNA（0.39 ng），为在可用DNA量有限的研究中使用该分析开辟了前景。
