基于适配体的荧光测定法用于直接从临床样本中鉴定耐甲氧西林金黄色葡萄球菌。
Aptamer-based fluorometric assay for direct identification of methicillin-resistant Staphylococcus aureus from clinical samples.
作者信息
Qiao Jinjuan, Meng Xiangying, Sun Yufang, Li Qian, Zhao Ronglan, Zhang Yun, Wang Jiaqi, Yi Zhengjun
机构信息
Department of Medical Laboratory, Weifang Medical University, Weifang, Shandong 261053, PR China; Key Laboratory of Clinical Laboratory Diagnostics in the Universities of Shandong Province, Weifang Medical University, Weifang, Shandong 261053, PR China; Institute of Nanomedicine Technology, Weifang Medical University, Weifang, Shandong 261053, PR China.
Department of Medical Laboratory, Weifang Medical University, Weifang, Shandong 261053, PR China; Key Laboratory of Clinical Laboratory Diagnostics in the Universities of Shandong Province, Weifang Medical University, Weifang, Shandong 261053, PR China; Institute of Nanomedicine Technology, Weifang Medical University, Weifang, Shandong 261053, PR China.
出版信息
J Microbiol Methods. 2018 Oct;153:92-98. doi: 10.1016/j.mimet.2018.09.011. Epub 2018 Sep 20.
Accurate and rapid identification of methicillin-resistant Staphylococcus aureus (MRSA) is of important clinical significance. In this study, a novel aptamer-based fluorometric assay was developed for detection of MRSA in clinical samples by coupling with immunomagnetic separation. The S. aureus cells in clinical specimens were enriched by magnetic separation. Following lysis by staphylococcal lysin, the PBP2a proteins were released from S. aureus cells and detected by the aptamer-based fluorometric assay. Without lengthy period of bacteria cultivation in the traditional susceptibility testing, this test has an overall testing time of only 2 h with the detection limit of 2.63 × 10 and 1.38 × 10 CFU/mL in PBS and spiked nasal swab, respectively. Since it is simple, rapid and sensitive, this method could be used for the detection of MRSA in various clinical samples.
准确快速地鉴定耐甲氧西林金黄色葡萄球菌（MRSA）具有重要的临床意义。在本研究中，通过与免疫磁分离相结合，开发了一种基于新型适体的荧光检测方法，用于检测临床样本中的MRSA。临床标本中的金黄色葡萄球菌细胞通过磁分离进行富集。经葡萄球菌溶素裂解后，PBP2a蛋白从金黄色葡萄球菌细胞中释放出来，并通过基于适体的荧光检测法进行检测。该检测无需传统药敏试验中漫长的细菌培养时间，总检测时间仅为2小时，在PBS和加标的鼻拭子中的检测限分别为2.63×10和1.38×10 CFU/mL。由于该方法简单、快速且灵敏，可用于检测各种临床样本中的MRSA。
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