Department of Pharmaceutics, Institute of Pharmacy, Nirma University, Ahmedabad, Gujarat 382481 India.
Australian Centre for Antimicrobial Resistance Ecology, School of Animal and Veterinary Sciences, The University of Adelaide, Roseworthy Campus, Roseworthy, South Australia 5371, Australia.
J Pharm Sci. 2019 Feb;108(2):1007-1016. doi: 10.1016/j.xphs.2018.09.011. Epub 2018 Sep 19.
Antimicrobial resistance at the infected site is a serious medical issue that increases patient morbidity and mortality. Silver has antibacterial activity associated with some dose-dependent toxicity. Silver nanoparticles, due to larger surface area, have antibacterial properties, which make them useful in the treatment of infections. Chitosan-stabilized silver nanoparticles (CH-AgNP) were formulated and evaluated for minimal inhibitory concentration and minimal bactericidal concentration testing against Staphylococcus aureus ATCC 29213, S aureus ATCC 25923, Pseudomonas aeruginosa ATCC 27853, Escherichia coli ATCC 25922, and 20 methicillin-resistant S aureus isolates. Minimum biofilm eradication concentration study was used to evaluate the biofilm reduction, and in vitro antimicrobial checkerboard assays were performed. The effective optimum ratio of AgNP:chitosan solution was 1:4. Minimal inhibitory concentration and minimal bactericidal concentration ranges of CH-AgNP were 4 to 14 times lower compared to AgNP alone against methicillin-resistant S aureus isolates. Minimum biofilm eradication concentration values of CH-AgNP for ATCC PA-01, P aeruginosa isolate 1, and P aeruginosa isolate 2 were found to be >84.59 μg/mL, 42.29 μg/mL, and 21.15 μg/mL, respectively. Thus, CH-AgNP is a potential formulation for wound treatment and management of infected sites associated with antimicrobial resistance.
感染部位的抗菌耐药性是一个严重的医学问题，会增加患者的发病率和死亡率。银具有与某些剂量相关的毒性相关的抗菌活性。由于表面积较大，纳米银颗粒具有抗菌特性，这使得它们在治疗感染方面非常有用。本文制备并评价了壳聚糖稳定的载银纳米颗粒（CH-AgNP）对金黄色葡萄球菌 ATCC 29213、金黄色葡萄球菌 ATCC 25923、铜绿假单胞菌 ATCC 27853、大肠埃希菌 ATCC 25922 和 20 株耐甲氧西林金黄色葡萄球菌分离株的最低抑菌浓度（MIC）和最低杀菌浓度（MBC）测试。采用最低生物膜清除浓度（MBEC）研究来评估生物膜减少情况，并进行了体外抗菌棋盘试验。AgNP：壳聚糖溶液的有效最佳比例为 1:4。与单独的 AgNP 相比，CH-AgNP 对耐甲氧西林金黄色葡萄球菌分离株的 MIC 和 MBC 范围低 4 至 14 倍。CH-AgNP 对 ATCC PA-01、铜绿假单胞菌分离株 1 和铜绿假单胞菌分离株 2 的最低生物膜清除浓度（MBEC）值分别为>84.59μg/mL、42.29μg/mL 和 21.15μg/mL。因此，CH-AgNP 是一种有潜力的治疗伤口和管理与抗菌耐药性相关感染部位的制剂。
