Laboratory of Oral Biomedical Science and Translational Medicine, School of Stomatology, Tongji University, Shanghai, China (mainland).
Department of Oral Surgery, Shanghai Stomatology Disease Centre, Shanghai, China (mainland).
Med Sci Monit. 2018 Sep 23;24:6695-6706. doi: 10.12659/MSM.910983.
BACKGROUND The aim of this study was to determine the role of icariin, a Chinese traditional herbal medicine extracted from Epimedium, in osteoarthritis (OA), using the murine anterior cruciate ligament transection (ACLT)-induced model of OA and micromass culture of murine chondrocytes. MATERIAL AND METHODS Twenty-four three-month-old C57/6J mice were randomly divided into three groups: the sham group (no surgery and joint injection with normal saline) (N=8); the ACLT + ICA group (ACLT surgery and icariin treatment) (N=8); and the ACLT group (ACLT surgery and joint injection with normal saline) (N=8). At 12 weeks after ACLT surgery, murine articular cartilage was harvested from all mice for histological evaluation of any differences in cartilage degeneration. In vitro micromass culture of mouse chondrocytes was used to study the effects of icariin on chondrocyte differentiation and growth from the three mouse groups. RESULTS Icariin treatment (mice in the ACLT + ICA group) significantly reduced degeneration of cartilage in OA with increased cartilage thickness, associated with increased expression of collagen type II alpha 1 (COL2A1), decreased chondrocyte hypertrophy, and decreased expression of collagen type X (ColX) and matrix metalloproteinase 13 (MMP13). In vitro, icariin promoted chondrocyte differentiation by upregulating the expression of agrrecan, Sox9 and parathyroid hormone-related protein (PHrP) and down-regulation of Indian hedgehog (Ihh) and genes regulated by Ihh. CONCLUSIONS In a mouse model of OA icariin treatment reduced destruction of cartilage, promoted chondrocyte differentiation, upregulated expression of PHrP and down-regulated the expression of Ihh.
本研究旨在通过使用鼠前交叉韧带切断（ACLT）诱导的骨关节炎（OA）模型和鼠软骨细胞微团培养来确定从淫羊藿中提取的中药淫羊藿苷在 OA 中的作用。
24 只 3 月龄 C57/6J 小鼠随机分为三组：假手术组（不手术，关节内注射生理盐水）（N=8）；ACLT+ICA 组（ACLT 手术和淫羊藿苷治疗）（N=8）；ACLT 组（ACLT 手术和关节内注射生理盐水）（N=8）。在 ACLT 手术后 12 周，从所有小鼠中取出关节软骨，用于评估软骨退变的差异。体外鼠软骨细胞微团培养用于研究淫羊藿苷对来自三组小鼠的软骨细胞分化和生长的影响。
淫羊藿苷治疗（ACLT+ICA 组小鼠）显著减轻 OA 软骨退变，增加软骨厚度，同时增加胶原 II 型α1（COL2A1）表达，减少软骨细胞肥大，降低胶原 X（ColX）和基质金属蛋白酶 13（MMP13）表达。体外，淫羊藿苷通过上调聚集蛋白聚糖、Sox9 和甲状旁腺激素相关蛋白（PHrP）的表达，下调印度刺猬（Ihh）和 Ihh 调节基因的表达，促进软骨细胞分化。
在 OA 小鼠模型中，淫羊藿苷治疗减少软骨破坏，促进软骨细胞分化，上调 PHrP 表达，下调 Ihh 表达。
