Kiyoi Takeshi
Division of Analytical Bio-Medicine, Advanced Research Support Center, Ehime University, Toon, Ehime, Japan.
Methods Mol Biol. 2018;1868:215-222. doi: 10.1007/978-1-4939-8802-0_22.
Bone homeostasis depends on balanced bone deposition and bone resorption, which are mediated by osteoblasts and osteoclasts, respectively. As one therapeutic strategy, the blockage of osteoclast activity reduces subsequent bone erosion. Morphological analysis of bone resorption pits formed by osteoclasts by using scanning electron microscope is an effective method for understanding rheumatoid arthritis. Here we describe methods for observing surface microstructure of pits formed by osteoclasts on hard tissue sections.
骨稳态依赖于成骨细胞和破骨细胞分别介导的骨沉积和骨吸收之间的平衡。作为一种治疗策略，阻断破骨细胞活性可减少随后的骨侵蚀。利用扫描电子显微镜对破骨细胞形成的骨吸收陷窝进行形态学分析是了解类风湿性关节炎的有效方法。在此，我们描述了观察硬组织切片上破骨细胞形成的陷窝表面微观结构的方法。
