Raff H, Skelton M M, Merrill D C, Cowley A W
Am J Physiol. 1986 Dec;251(6 Pt 2):R1235-9. doi: 10.1152/ajpregu.1986.251.6.R1235.
We recently reported that ovine corticotropin releasing factor (CRF) infusion in conscious dogs elevated plasma vasopressin. The present study examines the vasopressin, adrenocorticotropic hormone (ACTH), and cortisol responses to CRF infusion (20 ng X kg-1 X min-1), to hypertonic saline infusion (NaCl 0.054 meq X kg-1 X min-1), and to simultaneous coinfusion of CRF and NaCl (CRF + NaCl) without (no-dex) or with (dex-treated) dexamethasone pretreatment in six conscious dogs (6-8 experiments/dog). CRF had no significant effect on plasma sodium or osmolality, blood pressure, or heart rate. NaCl increased plasma sodium from 146 +/- 1 to 151 +/- 1 meq/l and plasma osmolality from 298 +/- 3 to 305 +/- 3 mosmol/kg. Vasopressin increased significantly during CRF (2.1 +/- 0.5 to 4.8 +/- 1.1 pg/ml) and NaCl (1.9 +/- 0.3 to 5.0 +/- 0.8 pg/ml). Coinfusion of CRF and NaCl resulted in a response larger than the sum of the two infusions alone (3.0 +/- 1.6 to 31.4 +/- 18.5 pg/ml). The ACTH response to CRF (45 +/- 8 to 288 +/- 88 pg/ml) was not augmented by coinfusion with NaCl. DEX attenuated the vasopressin and ACTH responses to each infusion. We conclude that CRF-induced increases in vasopressin are augmented by a simultaneous osmotic stimulus. In addition, the plasma vasopressin responses to CRF and/or hypertonic saline infusion are inhibited by glucocorticoid pretreatment.
我们最近报道，向清醒犬输注绵羊促肾上腺皮质激素释放因子（CRF）可使血浆血管加压素升高。本研究检测了6只清醒犬（每只犬进行6 - 8次实验）在输注CRF（20 ng·kg⁻¹·min⁻¹）、高渗盐水（0.054 meq·kg⁻¹·min⁻¹的NaCl）以及同时输注CRF和NaCl（CRF + NaCl）时，血管加压素、促肾上腺皮质激素（ACTH）和皮质醇的反应，实验分为未用（未用地塞米松）或用（地塞米松处理）地塞米松预处理两组。CRF对血浆钠、渗透压、血压或心率无显著影响。NaCl使血浆钠从146 ± 1 meq/L升高至151 ± 1 meq/L，血浆渗透压从298 ± 3 mosmol/kg升高至305 ± 3 mosmol/kg。在输注CRF（从2.1 ± 0.5至4.8 ± 1.1 pg/ml）和NaCl（从1.9 ± 0.3至5.0 ± 0.8 pg/ml）期间，血管加压素显著升高。同时输注CRF和NaCl导致的反应大于单独两种输注反应之和（从3.0 ± 1.6至31.4 ± 18.5 pg/ml）。与NaCl同时输注并未增强CRF引起的ACTH反应（从45 ± 8至288 ± 88 pg/ml）。地塞米松减弱了每种输注引起的血管加压素和ACTH反应。我们得出结论，CRF诱导的血管加压素升高可被同时的渗透压刺激增强。此外，糖皮质激素预处理可抑制血浆血管加压素对CRF和/或高渗盐水输注的反应。
