Department of Chemistry, Columbia University, 3000 Broadway, New York, NY 10027, USA.
Analyst. 2018 Oct 8;143(20):4844-4848. doi: 10.1039/c8an00910d.
Stimulated Raman Scattering (SRS) coupled with alkyne tags has been an emerging imaging technique to visualize small-molecule species with high sensitivity and specificity. Here we describe the development of a ratiometric Raman probe for visualizing hydrogen sulfide (H2S) species in living cells as the first alkyne-based sensor for SRS microscopy. This probe uses an azide unit as a selective reactive site, and it targets mitochondria with high specificity. The SRS ratiometric images show a strong response to H2S level changes in living cells.
受激拉曼散射(SRS)与炔烃标签相结合,已经成为一种新兴的成像技术,可以高灵敏度和特异性地可视化小分子物质。在这里,我们描述了一种用于在活细胞中可视化硫化氢(H2S)物质的比率型拉曼探针的开发,这是第一个基于炔烃的 SRS 显微镜传感器。该探针使用叠氮基团作为选择性反应位点,并且具有高特异性靶向线粒体。SRS 比率成像显示出对活细胞中 H2S 水平变化的强烈响应。