Key Laboratory for Advanced Materials, and Department of Chemistry, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237 (China).
Angew Chem Int Ed Engl. 2015 Oct 19;54(43):12758-61. doi: 10.1002/anie.201505025. Epub 2015 Aug 28.
Hydrogen sulfide (H2 S) has emerged as an important gasotransmitter in diverse physiological processes, although many aspects of its roles remain unclear, partly owing to a lack of robust analytical methods. Herein we report a novel surface-enhanced Raman scattering (SERS) nanosensor, 4-acetamidobenzenesulfonyl azide-functionalized gold nanoparticles (AuNPs/4-AA), for detecting the endogenous H2 S in living cells. The detection is accomplished with SERS spectrum changes of AuNPs/4-AA resulting from the reaction of H2 S with 4-AA on AuNPs. The SERS nanosensor exhibits high selectivity toward H2 S. Furthermore, AuNPs/4-AA responds to H2 S within 1 min with a 0.1 μM level of sensitivity. In particular, our SERS method can be utilized to monitor the endogenous H2 S generated in living glioma cells, demonstrating its great promise in studies of pathophysiological pathways involving H2 S.
硫化氢（H2S）已成为多种生理过程中一种重要的气体信号分子，但其作用的许多方面仍不清楚，部分原因是缺乏稳健的分析方法。在此，我们报告了一种新型的表面增强拉曼散射（SERS）纳米传感器，即 4-乙酰氨基苯磺酰叠氮功能化金纳米粒子（AuNPs/4-AA），用于检测活细胞内的内源性 H2S。该检测是通过 AuNPs/4-AA 的 SERS 光谱变化来实现的，这是由于 H2S 与 AuNPs 上的 4-AA 反应所致。该 SERS 纳米传感器对 H2S 表现出高选择性。此外，AuNPs/4-AA 在 1min 内对 H2S 做出响应，其灵敏度达到 0.1μM 水平。特别是，我们的 SERS 方法可用于监测活神经胶质瘤细胞中产生的内源性 H2S，这表明它在涉及 H2S 的病理生理途径研究中具有广阔的应用前景。
