Azizkhan J C, Vaughn J P, Christy R J, Hamlin J L
Biochemistry. 1986 Oct 7;25(20):6228-36. doi: 10.1021/bi00368a059.
We have sequenced the 1240 base pairs (bp) upstream from the translation start site of the hamster dihydrofolate reductase (DHFR) gene. The DNA in the 5' flanking region contains several elements that are homologous in both sequence and relative location to corresponding elements in the human and murine DHFR genes: an 11-bp element adjacent to the ATG codon, a 19-bp element that coincides with the major transcription start site, and two 29-bp upstream elements that are represented 4 times in the murine DHFR gene but only once in the human gene. Two clusters of short, G/C-rich elements conforming to the consensus binding sequence for the transcription factor Spl are located in the upstream region in all three genes. The symmetrical placement of the G/C boxes coincides with a symmetrical DNase I hypersensitive pattern in the chromatin, suggesting that the Spl protein may be involved in maintaining chromatin structure in this region.
我们已对仓鼠二氢叶酸还原酶（DHFR）基因翻译起始位点上游的1240个碱基对（bp）进行了测序。5'侧翼区域的DNA包含几个在序列和相对位置上与人类和小鼠DHFR基因中的相应元件同源的元件：一个与ATG密码子相邻的11bp元件，一个与主要转录起始位点重合的19bp元件，以及两个29bp的上游元件，它们在小鼠DHFR基因中出现4次，但在人类基因中仅出现一次。符合转录因子Spl共有结合序列的两个富含G/C的短元件簇位于所有三个基因的上游区域。G/C盒的对称排列与染色质中对称的DNase I超敏模式一致，表明Spl蛋白可能参与维持该区域的染色质结构。
