二丁酰环磷腺苷对离体大鼠肝细胞牛磺胆酸排泄的影响。

The effect of dibutyryl cyclic AMP on the excretion of taurocholic acid from isolated rat liver cells.

作者信息

Botham K M, Suckling K E

出版信息

Biochim Biophys Acta. 1986 Dec 19;889(3):382-5. doi: 10.1016/0167-4889(86)90202-8.

Abstract

Dibutyryl cyclic AMP (50-1000 microM) was found to increase the initial rate of efflux of taurocholic acid from isolated rat hepatocytes. Efflux of the bile acid was inhibited by sodium, and in the absence of sodium dibutyryl cyclic AMP failed to stimulate the rate. Increasing the concentration of calcium from 0 to 1.2 mM had no effect on the initial rate of taurocholic acid efflux from the cells, but the absence of calcium markedly reduced the effect of dibutyryl cyclic AMP. The results suggest that changes in the fluxes of sodium and calcium are involved in the effect of the cyclic nucleotide on taurocholic acid efflux from the cells.

摘要

已发现二丁酰环磷酸腺苷（50 - 1000微摩尔）可提高牛磺胆酸从分离的大鼠肝细胞流出的初始速率。胆汁酸的流出受到钠的抑制，并且在无钠的情况下，二丁酰环磷酸腺苷无法刺激该速率。将钙浓度从0提高到1.2毫摩尔对细胞中牛磺胆酸的初始流出速率没有影响，但无钙会显著降低二丁酰环磷酸腺苷的作用。结果表明，钠和钙通量的变化参与了环核苷酸对细胞中牛磺胆酸流出的影响。