Poomarimuthu Maheshkumar, Elango Sivakumar, Solomon Pravin Raj, Soundarapandian Sambath, Mariakuttikan Jayalakshmi
a Department of Immunology , School of Biological Sciences, Madurai Kamaraj University , Madurai , Tamil Nadu, India.
b Institute of Child Health and Research Centre , Government Rajaji Hospital , Madurai , Tamil Nadu, India.
Fetal Pediatr Pathol. 2018 Oct;37(5):309-318. doi: 10.1080/15513815.2018.1494232. Epub 2018 Sep 24.
Proinflammatory and anti-inflammatory cytokines play a crucial role in the development and maintenance of immune mediated inflammatory diseases including rheumatic heart disease (RHD). Polymorphisms in tumor necrosis factor (TNF)-α, interferon (IFN)-γ, and interleukin (IL)-10 genes influence the differential cytokine expression as well as the pathogenesis of various inflammatory and autoimmune diseases.
The aim of the study is to investigate the association between TNF-α, IFN-γ, and IL-10 gene polymorphisms and RHD in South Indian population.
TNF-α (-308, -238), IFN-γ (+874), and IL-10 (-1082, -819, -592) gene polymorphisms were determined in 100 patients with RHD and 127 healthy siblings by PCR.
There was no significant difference in the genotype, allele, and haplotype frequencies of TNF-α, IFN-γ, and IL-10 polymorphisms between RHD patients and healthy siblings.
The present study suggests that TNF-α, IFN-γ, and IL-10 gene variants may not be associated with the development of RHD in South Indian population.
促炎细胞因子和抗炎细胞因子在包括风湿性心脏病（RHD）在内的免疫介导性炎症疾病的发生和维持中起关键作用。肿瘤坏死因子（TNF）-α、干扰素（IFN）-γ和白细胞介素（IL）-10基因的多态性影响细胞因子的差异表达以及各种炎症和自身免疫性疾病的发病机制。
本研究旨在调查南印度人群中TNF-α、IFN-γ和IL-10基因多态性与风湿性心脏病的关联。
采用聚合酶链反应（PCR）检测100例风湿性心脏病患者和127名健康同胞的TNF-α（-308、-238）、IFN-γ（+874）和IL-10（-1082、-819、-592）基因多态性。
风湿性心脏病患者与健康同胞之间，TNF-α、IFN-γ和IL-10多态性的基因型、等位基因和单倍型频率无显著差异。
本研究表明，在南印度人群中，TNF-α、IFN-γ和IL-10基因变异可能与风湿性心脏病的发生无关。
