Kishimoto C, Kuribayashi K, Masuda T, Tomioka N, Kawai C
Cardiovasc Res. 1986 Oct;20(10):768-73. doi: 10.1093/cvr/20.10.768.
To clarify the role of the immune system in the development of myocarditis, BALB/c-nu/nu mice (group 1), BALB/c-nu/+ mice (group 2), BALB/c-nu/nu mice injected with 5 X 10(7) spleen cells from BALB/c-nu/+ mice (group 3), and BALB/c-nu/nu mice injected with 5 X 10(7) spleen cells from BALB/c-nu/+ mice treated with rat anti-Thy-1.2 monoclonal antibody with complement (group 4) were inoculated with encephalomyocarditis virus. There were no significant differences in the incidence of myocarditis among the four groups. Virus titrations of the heart and serum neutralising antibody titres in the four groups did not show any significant differences. Fifty two per cent (26/50) of group 2 and 43% (20/46) of group 3 died on days 9-15, when congestive heart failure developed. Only 9% (5/54) of group 1 and 8% (1/12) of group 4, however, died on days 9-15. Pathological examination confirmed congestive heart failure in groups 2 and 3 but not in groups 1 and 4. Dilatation of the ventricular cavities, pleural effusion, ascites, and congestion of the lungs and liver were present in groups 2 and 3 but not in groups 1 and 4. Cellular infiltration and myocardial necrosis were severe in groups 2 and 3 but minimal in groups 1 and 4. Thus the severity of myocarditis may be regulated by T cells. So-called silent myocarditis seen in clinical settings may be similar to myocarditis in BALB/c-nu/nu mice.
为阐明免疫系统在心肌炎发展中的作用，将BALB/c-nu/nu小鼠（第1组）、BALB/c-nu/+小鼠（第2组）、注射5×10⁷个来自BALB/c-nu/+小鼠脾细胞的BALB/c-nu/nu小鼠（第3组）以及注射5×10⁷个来自经大鼠抗Thy-1.2单克隆抗体与补体处理的BALB/c-nu/+小鼠脾细胞的BALB/c-nu/nu小鼠（第4组）接种脑心肌炎病毒。四组之间心肌炎的发病率无显著差异。四组心脏的病毒滴定和血清中和抗体滴度均未显示出任何显著差异。第2组的52%（26/50）和第3组的43%（20/46）在第9至15天出现充血性心力衰竭时死亡。然而，第1组仅9%（5/54）和第4组仅8%（1/12）在第9至15天死亡。病理检查证实第2组和第3组存在充血性心力衰竭，而第1组和第4组没有。第2组和第3组出现心室腔扩张、胸腔积液、腹水以及肺和肝充血，而第1组和第4组没有。第2组和第3组的细胞浸润和心肌坏死严重，而第1组和第4组则很轻微。因此，心肌炎的严重程度可能受T细胞调节。临床环境中所见的所谓隐匿性心肌炎可能与BALB/c-nu/nu小鼠的心肌炎相似。
