American Heart Association - Tobacco Regulation and Addiction Center, Superfund Research Center and Diabetes and Obesity Center, Institute of Molecular Cardiology, University of Louisville, 580 South Preston Street, Louisville, KY, 40202, United States of America.
American Heart Association - Tobacco Regulation and Addiction Center, Superfund Research Center and Diabetes and Obesity Center, Institute of Molecular Cardiology, University of Louisville, 580 South Preston Street, Louisville, KY, 40202, United States of America; Department of Chemistry, University of Louisville, 2320 S. Brook St., Louisville, KY, 40292, United States of America.
J Chromatogr B Analyt Technol Biomed Life Sci. 2018 Nov 1;1099:83-91. doi: 10.1016/j.jchromb.2018.09.012. Epub 2018 Sep 12.
Biogenic monoamines, including catecholamines and serotonin are important hormones and neurotransmitters. Abnormal urinary levels of biogenic monoamines and their metabolites are associated with smoking, neuroendocrine tumors, as well as neurological and cardiovascular diseases. Measurements of free biogenic monoamines and their metabolites have been challenging because of low concentrations in complex biological matrices. Current methods require extensive enrichment and removal of interfering substances and can analyze only basic or acidic compounds in a single run. We developed a simple and robust dilute-and-shoot method capable of measuring 10 analytes, including free biogenic monoamines and their metabolites in human urine. The assay enables sensitive measurements of analytes within expected sample concentration ranges. To assess the assay's efficacy, we measured urinary levels of free biogenic monoamines and their metabolites in 255 non-smokers and 191 smokers. Our data show that while smokers had significantly higher urinary levels of free catecholamines and metanephrines, there was a decrease in levels of biogenic amine metabolites synthesized through the monoamine oxidase pathway - homovanillic acid and vanillylmandelic acid. The method could be used for high throughput measurement of the range of free biogenic amines and their metabolites in urine under a variety of different conditions.
生物源单胺类物质，包括儿茶酚胺和 5-羟色胺，是重要的激素和神经递质。生物源单胺类物质及其代谢物的尿液水平异常与吸烟、神经内分泌肿瘤以及神经和心血管疾病有关。由于在复杂的生物基质中浓度较低，因此对游离生物源单胺类物质及其代谢物的测量具有挑战性。目前的方法需要广泛的富集和去除干扰物质，并且只能在单次运行中分析基本或酸性化合物。我们开发了一种简单而强大的稀释和注入方法，能够测量 10 种分析物，包括人尿中的游离生物源单胺类物质及其代谢物。该测定法能够在预期的样品浓度范围内对分析物进行灵敏的测量。为了评估该测定法的功效，我们测量了 255 名不吸烟者和 191 名吸烟者的尿中游离生物源单胺类物质及其代谢物的水平。我们的数据表明，虽然吸烟者的尿中游离儿茶酚胺和甲肾上腺素水平显著升高，但通过单胺氧化酶途径合成的生物胺代谢物水平下降，即香草扁桃酸和 4-羟基-3-甲氧基扁桃酸。该方法可用于在各种不同条件下对尿中游离生物源单胺类物质及其代谢物的范围进行高通量测量。
