牺牲模板法合成聚吡咯中空纤维用于光热治疗。
Sacrificial template-based synthetic approach of polypyrrole hollow fibers for photothermal therapy.
机构信息
Department of Bionanosystem Engineering, Graduate School, Chonbuk National University, Jeonju 561-756, Republic of Korea; Department of Chemistry, Amrit Campus, Tribhuvan University, Kathmandu, Nepal.
Department of Bionanosystem Engineering, Graduate School, Chonbuk National University, Jeonju 561-756, Republic of Korea.
出版信息
J Colloid Interface Sci. 2019 Jan 15;534:447-458. doi: 10.1016/j.jcis.2018.09.047. Epub 2018 Sep 17.
In the present work, polypyrrole hollow fibers (PPy-HFs) were fabricated by sacrificial removal of soft templates of electrospun polycaprolactone (PCL) fibers with polypyrrole (PPy) coating through chemical polymerization of pyrrole monomer. Different physicochemical properties of as-fabricated PPy-HFs were then studied by Field emission scanning electron microscopy (FE-SEM), X-ray diffraction (XRD), Fourier transform infra-red (FT-IR) spectroscopy, Differential scanning calorimetry/Thermogravimetric analysis (DSC/TGA), and X-ray photoelectron spectroscopy (XPS). The photothermal activity of PPy-HF was studied by irradiating 808-nm near infra-red (NIR) light under different power values with various concentrations of PPy-HFs dispersed in phosphate buffer solution (PBS, pH 7.4). These PPy-HFs exhibited enhanced photothermal performance compared with polypyrrole nanoparticles (PPy-NPs). Furthermore, these PPy-HFs showed photothermal effect that was laser-power- and concentration-dependent. The photothermal toxicity of the resulting nanofiber was evaluated using cell counting kit-8 (CCK-8) and live and dead cell assays. Results showed that these PPy-HFs were more effective in killing cancer cells under NIR irradiation. In contrast, hollow-fiber showed no cytotoxicity without NIR exposure. Among different nanofiber formulations, PPy-160 exhibited the highest photothermal toxicity. It could be explained by its enhanced photothermal performance compared to other specimens. The resulting PPy-HFs showed superior drug-loading capacity to PPy-NPs. This might be attributed to adequate binding of the drug into both luminal and abluminal hollow-fiber surfaces. Fabrication of this substrate type opens a promising new avenue for architectural design of biocompatible organic polymer for biomedical field.
在本工作中，通过聚吡咯单体的化学聚合，采用牺牲模板法去除静电纺丝聚己内酯（PCL）纤维的软模板，制备了聚吡咯中空纤维（PPy-HF）。然后通过场发射扫描电子显微镜（FE-SEM）、X 射线衍射（XRD）、傅里叶变换红外（FT-IR）光谱、差示扫描量热/热重分析（DSC/TGA）和 X 射线光电子能谱（XPS）研究了所制备的 PPy-HF 的不同理化性质。在磷酸盐缓冲溶液（PBS，pH 7.4）中，用不同浓度的 PPy-HF 分散体，在不同功率值下用 808nm 近红外（NIR）光照射，研究了 PPy-HF 的光热活性。与聚吡咯纳米粒子（PPy-NPs）相比，这些 PPy-HF 表现出增强的光热性能。此外，这些 PPy-HF 表现出与激光功率和浓度相关的光热效应。用细胞计数试剂盒-8（CCK-8）和活死细胞检测评估所得纳米纤维的光热毒性。结果表明，在 NIR 照射下，这些 PPy-HF 更有效地杀死癌细胞。相比之下，在没有 NIR 暴露的情况下，中空纤维没有细胞毒性。在不同的纳米纤维配方中，PPy-160 表现出最高的光热毒性。这可以通过其与其他标本相比增强的光热性能来解释。所得的 PPy-HF 表现出比 PPy-NPs 更高的载药能力。这可能归因于药物充分结合到内腔和外腔中空纤维表面。这种基底类型的制造为生物医学领域中生物相容性有机聚合物的结构设计开辟了一条有前途的新途径。
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