Murray J L, Bywaters D W, Reuben J M, Mansell P W, Hersh E M
Clin Immunol Immunopathol. 1987 Jan;42(1):10-7. doi: 10.1016/0090-1229(87)90168-1.
The purine metabolic enzymes adenosine deaminase (ADA), purine nucleoside phosphorylase (PNP), and 5'nucleotidase (5NT) have been shown to be important for normal lymphocyte maturation. Abnormalities of these enzymes have been associated with hereditary as well as acquired immunodeficiency states. Enzyme activity was measured in helper (OKT4) and suppressor (OKT8) lymphocyte subsets from 10 homosexuals with AIDS-related complex (ARC) and in 10 healthy controls. There were no significant differences in either mean ADA activity or mean PNP activity between ARC OKT4 cells and control OKT4 cells and between ARC OKT8 cells and control OKT8 cells. By contrast, mean 5NT activity was slightly decreased in OKT4 cells from ARC patients compared with that of controls and more significantly diminished in ARC OKT8 cells compared with that of controls. Both deoxyadenosine and deoxyguanosine, when incubated separately with OKT4 and OKT8 cells in the presence of EHNA, an ADA inhibitor, did not significantly inhibit lymphocyte blastogenesis to a greater extent in ARC patients than in controls. Hence, the decreases in 5NT activity most likely reflect lymphocyte immaturity and are not associated with biochemical abnormalities leading to increased deoxynucleoside toxicity.
嘌呤代谢酶腺苷脱氨酶（ADA）、嘌呤核苷磷酸化酶（PNP）和5'核苷酸酶（5NT）已被证明对正常淋巴细胞成熟很重要。这些酶的异常与遗传性以及获得性免疫缺陷状态有关。在10名患有艾滋病相关综合征（ARC）的同性恋者和10名健康对照者的辅助性（OKT4）和抑制性（OKT8）淋巴细胞亚群中测量了酶活性。ARC组OKT4细胞与对照组OKT4细胞之间以及ARC组OKT8细胞与对照组OKT8细胞之间，平均ADA活性或平均PNP活性均无显著差异。相比之下，与对照组相比，ARC患者的OKT4细胞中平均5NT活性略有降低，而ARC组OKT8细胞中的平均5NT活性与对照组相比则显著降低。在存在ADA抑制剂EHNA的情况下，分别将脱氧腺苷和脱氧鸟苷与OKT4和OKT8细胞一起孵育时，ARC患者的淋巴细胞母细胞生成受到的抑制程度并不比对照组更显著。因此，5NT活性的降低很可能反映了淋巴细胞的不成熟，并且与导致脱氧核苷毒性增加的生化异常无关。
