Bevington A, Mundy K I, Yates A J, Kanis J A, Russell R G, Taylor D J, Rajagopalan B, Radda G K
Clin Sci (Lond). 1986 Dec;71(6):729-35. doi: 10.1042/cs0710729.
In order to study the relationship between extracellular and intracellular concentrations of orthophosphate (Pi), phosphorus nuclear magnetic resonance spectra were recorded, at rest, from the flexor digitorum superficialis muscle of hypophosphataemic patients with vitamin D-resistant rickets, and patients with Paget's disease of bone before and after they had been made hyperphosphataemic by treatment with the drug ethylidene-1-hydroxy-1,1-bisphosphonate. Changes in intramuscular P1 were estimated from the ratio of the areas of the Pi to adenosine 5'-triphosphate peaks. Even though the plasma Pi concentration in these patients spanned a fourfold range (0.5-2.0 mmol/l) the corresponding intramuscular Pi concentration increased by only 70%. A similar effect was observed in erythrocytes, from patients with these disorders, which were incubated in autologous plasma at 37 degrees C, under an atmosphere of O2 + CO2 (95:5, v/v). However, chloride ions, which are transported passively across the cell membrane, showed no change in distribution between cells and plasma, indicating that there was no general effect on passive anion distribution. When erythrocytes from normal subjects were incubated in autologous plasma (1.0 mmol of Pi/l) and in plasma supplemented with Pi (2.3 mmol of Pi/l), the Pi concentration in the cells, at steady state, increased only from 0.57 to 0.78 mmol/l cells, suggesting that the effect was not an artifact of disease or drug therapy. It is concluded that, in human skeletal myocytes and erythrocytes, the percentage change in the concentration of cytoplasmic Pi is lower than that in plasma. This implies that these cells can buffer or regulate cytoplasmic Pi when the extracellular concentration is disturbed.
为了研究细胞外与细胞内正磷酸盐（Pi）浓度之间的关系，对患有维生素D抵抗性佝偻病的低磷血症患者以及患有佩吉特骨病的患者，在使用药物亚乙基 - 1 - 羟基 - 1,1 - 二膦酸盐治疗使其血磷升高之前和之后，对其指浅屈肌进行了静息状态下的磷核磁共振光谱记录。通过Pi与腺苷5'-三磷酸峰面积之比估算肌肉内P1的变化。尽管这些患者的血浆Pi浓度范围跨度为四倍（0.5 - 2.0 mmol/l），但相应的肌肉内Pi浓度仅增加了70%。在患有这些疾病的患者的红细胞中也观察到了类似的效应，这些红细胞在37℃、O2 + CO2（95:5，v/v）气氛下于自体血浆中孵育。然而，被动跨细胞膜转运的氯离子在细胞与血浆之间的分布没有变化，这表明对被动阴离子分布没有普遍影响。当将正常受试者的红细胞在自体血浆（1.0 mmol Pi/l）和补充了Pi的血浆（2.3 mmol Pi/l）中孵育时，细胞内Pi浓度在稳态下仅从0.57 mmol/l细胞增加到0.78 mmol/l细胞，这表明该效应不是疾病或药物治疗造成的假象。得出的结论是，在人类骨骼肌细胞和红细胞中，细胞质Pi浓度的百分比变化低于血浆中的变化。这意味着当细胞外浓度受到干扰时，这些细胞可以缓冲或调节细胞质Pi。
