Lei Zhentian, Kranawetter Clayton, Sumner Barbara W, Huhman David, Wherritt Daniel J, Thomas Andrew L, Rohla Charles, Sumner Lloyd W
University of Missouri Metabolomics Center, Columbia, MO 652111, USA.
Department of Biochemistry, University of Missouri, Columbia, MO 652111, USA.
Metabolites. 2018 Sep 23;8(4):56. doi: 10.3390/metabo8040056.
UHPLC-MS-based non-targeted metabolomics was used to investigate the biochemical basis of pecan scab resistance. Two contrasting pecan varieties, Kanza (scab-resistant) and Pawnee (scab-susceptible), were profiled and the metabolomics data analyzed using multivariate statistics. Significant qualitative and quantitative metabolic differences were observed between the two varieties. Both varieties were found to have some unique metabolites. Metabolites that were only present or more abundant in Kanza relative to Pawnee could potentially contribute to the scab resistance in Kanza. Some of these metabolites were putatively identified as quercetin derivatives using tandem mass spectrometry. This suggests that quercetin derivatives could be important to pecan scab resistance.
基于超高效液相色谱-质谱联用的非靶向代谢组学被用于研究山核桃对疮痂病抗性的生化基础。对两个对比鲜明的山核桃品种——抗疮痂病的“坎扎”和易感疮痂病的“波尼”进行了分析，并使用多元统计方法对代谢组学数据进行了分析。在两个品种之间观察到了显著的定性和定量代谢差异。发现两个品种都有一些独特的代谢物。相对于“波尼”，仅在“坎扎”中存在或含量更高的代谢物可能有助于“坎扎”对疮痂病的抗性。其中一些代谢物通过串联质谱法被初步鉴定为槲皮素衍生物。这表明槲皮素衍生物可能对山核桃对疮痂病的抗性很重要。
