Köhler G, Bässler R
Dtsch Med Wochenschr. 1986 Dec 19;111(51-52):1954-60. doi: 10.1055/s-2008-1068742.
Comparative biochemical and immunohistochemical investigations on receptors were performed on 100 breast cancers of different types. The biochemical analysis was carried out by means of radioimmunoassay (RIA) and immunohistochemical detection was performed with monoclonal antibodies against nuclear receptor protein (ER-ICA, Abbott Lab.). The semiquantitative evaluation is based on a histological score considering a graduated staining intensity and the percentage of positively reacting cells. The results of the two methods corresponded in 92% in positive and in 68% in negative receptor detection. The age-dependence is confirmed. The nuclear grading and histological grading show that the number of estrogen receptors gradually decreases with increasing degree of malignancy of the carcinomas. The advantage of immunohistochemical receptor assay consists in the microscopic investigation of homogeneous and heterogeneous degrees of intensity as well as in the possibility of determining receptor status in very small breast cancers, in punch biopsies and in tumours in which the tumour parenchyma is largely necrotic, sclerosed or surrounded by large amounts of mucus.
对100例不同类型的乳腺癌进行了受体的比较生化和免疫组化研究。生化分析采用放射免疫测定法（RIA），免疫组化检测使用抗核受体蛋白的单克隆抗体（ER-ICA，雅培实验室）。半定量评估基于组织学评分，考虑染色强度分级和阳性反应细胞的百分比。两种方法在受体检测阳性方面的结果相符率为92%，阴性方面为68%。年龄依赖性得到证实。核分级和组织学分级表明，随着癌恶性程度的增加，雌激素受体数量逐渐减少。免疫组化受体检测的优点在于能够对强度的均匀和不均匀程度进行显微镜检查，以及能够确定非常小的乳腺癌、穿刺活检组织和肿瘤实质大量坏死、硬化或被大量黏液包围的肿瘤中的受体状态。
