Charpin C, Martin P M, Jacquemier J, Lavaut M N, Pourreau-Schneider N, Toga M
Cancer Res. 1986 Aug;46(8 Suppl):4271s-4277s.
An estrogen receptor (ER) immunocytochemical assay (ER-ICA) was applied to 115 malignant breast carcinomas and the results were compared to those of steroid binding assays performed on cytosol extracts of the same tumors. Immunoperoxidase (peroxidase-antiperoxidase) staining was performed on frozen sections using rat monoclonal antibody to estrogen receptor H222SP gamma. A preembedding method was used for the immunoelectron microscopy study. A semiquantitative analysis and a computerized image analysis system (SAMBA 200 TITN) were used to evaluate the positive ER immunostaining. Positive immunostaining (81 of 115) was always located in the nucleus of tumor cells and of normal cells in adjacent breast tissue. The immunostaining pattern differed from one tumor to another, due to variations in either the intensity or the percentage of positive cells. When immunohistochemical staining was correlated to biochemical assay, there was an 88% correlation, and staining intensity and percentage of positive cells significantly increased (P less than 0.01) with cytosolic ER levels and were independent of cellularity. These results indicated that ER-ICA is to date the most reliable histochemical method for ER detection and correlated in 88% of the cases with ER biochemical assay; ER-ICA constitutes a method particularly valuable to screen ER negative tumors on condition that tumor fragment quality (sampling and storage) is perfectly controlled; ER-ICA provides additional information for heterogeneous ER distribution within tumors; ER-ICA as a qualitative method is unable to replace the quantitative ER determination obtained with biochemical assay although the computerized system (SAMBA 200) for image analysis of microscopic preparations constitutes a valuable improvement of immunostaining analysis; and ER-ICA based on ER antigenic site detection is complementary to biochemical assay based on ER functional site determination.
对115例乳腺恶性肿瘤进行了雌激素受体（ER）免疫细胞化学检测（ER-ICA），并将结果与对同一肿瘤细胞溶胶提取物进行的类固醇结合检测结果进行比较。使用大鼠抗雌激素受体单克隆抗体H222SPγ对冰冻切片进行免疫过氧化物酶（过氧化物酶-抗过氧化物酶）染色。免疫电子显微镜研究采用包埋前方法。使用半定量分析和计算机图像分析系统（SAMBA 200 TITN）评估ER免疫染色阳性情况。阳性免疫染色（115例中的81例）始终位于肿瘤细胞和相邻乳腺组织正常细胞的细胞核中。由于阳性细胞强度或百分比的差异，不同肿瘤的免疫染色模式有所不同。当免疫组织化学染色与生化检测相关联时，两者的相关性为88%，且染色强度和阳性细胞百分比随细胞溶胶ER水平显著增加（P<0.01），并且与细胞密度无关。这些结果表明，迄今为止，ER-ICA是检测ER最可靠的组织化学方法，在88% 的病例中与ER生化检测结果相关；只要肿瘤组织块质量（采样和储存）得到完美控制，ER-ICA对于筛查ER阴性肿瘤是一种特别有价值的方法；ER-ICA可提供肿瘤内ER异质性分布的额外信息；尽管用于显微镜标本图像分析的计算机系统（SAMBA 200）对免疫染色分析有很大改进，但作为一种定性方法，ER-ICA无法替代通过生化检测获得的ER定量测定；基于ER抗原位点检测的ER-ICA与基于ER功能位点测定的生化检测互为补充。
