Romanowska E, Gamian A, Dabrowski J
Eur J Biochem. 1986 Dec 15;161(3):557-64. doi: 10.1111/j.1432-1033.1986.tb10478.x.
The core structure of Citrobacter PCM 1487 lipopolysaccharide has been established using methylation analysis/mass spectrometry, chemical degradations and one- and two-dimensional 1H-NMR spectroscopy at 500 MHz. 1H-NMR assignments are given for all sugar components of the core oligosaccharide. In the formula shown below, the alternative locations of branch terminal heptose (LDHep) and diphosphorylethanolamine (PPEtN) residues are marked by dashed lines; dOclA stands for 3-deoxy-D-manno-octulosonic acid. (Formula: see text). The sample of the core oligosaccharide showed some microheterogeneity due to a slightly incomplete substitution by terminal N-acetylgalactosamine and a partial splitting of diphosphorylethanolamine residues.
利用甲基化分析/质谱、化学降解以及500兆赫的一维和二维氢核磁共振光谱法，已确定了柠檬酸杆菌PCM 1487脂多糖的核心结构。给出了核心寡糖所有糖成分的氢核磁共振归属。在下面所示的分子式中，分支末端庚糖（LDHep）和二磷酸乙醇胺（PPEtN）残基的交替位置用虚线表示；dOclA代表3-脱氧-D-甘露糖辛酸。（分子式：见正文）。核心寡糖样品显示出一些微观不均一性，这是由于末端N-乙酰半乳糖胺的取代略有不完全以及二磷酸乙醇胺残基的部分分裂所致。
