Saffold 卡波西病毒 2A-2B 交界处核糖体框架移位区的重组。

Recombination Located over 2A-2B Junction Ribosome Frameshifting Region of Saffold Cardiovirus.

机构信息

Institute of Tropical Medicine, University of São Paulo, São Paulo 05403-000, Brazil.

Enteric Disease Laboratory, Virology Center, Adolfo Lutz Institute, São Paulo 01246-000, Brazil.

出版信息

Viruses. 2018 Sep 24;10(10):520. doi: 10.3390/v10100520.

DOI:10.3390/v10100520

PMID:30249971

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6213509/

Abstract

Here we report the nearly full-length genome of a recombinant Saffold virus strain (SAFV-BR-193) isolated from a child with acute gastroenteritis. Evolutionary analysis performed using all available near-full length Saffold picornavirus genomes showed that the breakpoint found in the Brazilian strain (SAFV-BR-193) is indeed a recombination hotspot. Notably, this hotspot is located just one nucleotide after the ribosomal frameshift GGUUUUU motif in the SAFV genome. Empirical studies will be necessary to determine if this motif also affects the binding affinity of RNA-dependent RNA-polymerase (RdRp) and therefore increases the changes of RdRp swap between molecules during the synthesis of viral genomes.

摘要

我们在此报告了一株从急性肠胃炎患儿中分离得到的重组萨菲病毒（SAFV-BR-193）的近乎全长基因组。使用所有可获得的近乎全长萨菲病毒小核糖核酸病毒基因组进行的进化分析表明，在巴西分离株（SAFV-BR-193）中发现的断点确实是一个重组热点。值得注意的是，该热点正好位于萨菲病毒基因组中核糖体移码 GGUUUUU 基序后一个核苷酸处。需要进行实证研究以确定该基序是否也会影响 RNA 依赖性 RNA 聚合酶（RdRp）的结合亲和力，从而增加 RdRp 在病毒基因组合成过程中在分子间交换的可能性。