一种改进的酶联免疫吸附测定（ELISA）方法，该方法使用链霉亲和素-生物素复合物，用于检测感染鸡羽毛尖端中的马立克氏病病毒抗原。

An improved ELISA method, using a streptavidin-biotin complex, for detecting Marek's disease virus antigens in feather-tips of infected chickens.

作者信息

Davidson I, Malkinson M, Strenger C, Becker Y

出版信息

J Virol Methods. 1986 Nov;14(3-4):237-41. doi: 10.1016/0166-0934(86)90025-x.

Abstract

To improve sensitivity in the detection of Marek's disease virus (MDV) antigens in extracts of feather tips from infected chickens, we added a preformed streptavidin-biotin complex to the standard enzyme-linked immunosorbent assay (ELISA). Rabbit anti-chicken IgG-alkaline phosphatase that is used in the standard ELISA as the conjugate was replaced by a biotinylated rabbit anti-chicken IgG plus the streptavidin-biotin peroxidase complex (ABC) system. The ABC-ELISA system was correlated to the standard ELISA. There was increased sensitivity in the detection of MDV antigens present at low concentrations, while at the higher concentrations detection was similar to that in the standard ELISA. Both ELISA systems had the same increased sensitivity when compared with that of the agar gel precipitation (AGP) test.

摘要

为提高在感染鸡羽毛尖提取物中检测马立克氏病病毒（MDV）抗原的灵敏度，我们在标准酶联免疫吸附测定（ELISA）中添加了预先形成的链霉亲和素 - 生物素复合物。标准ELISA中用作缀合物的兔抗鸡IgG - 碱性磷酸酶被生物素化的兔抗鸡IgG加上链霉亲和素 - 生物素过氧化物酶复合物（ABC）系统所取代。ABC - ELISA系统与标准ELISA相关。在检测低浓度存在的MDV抗原时灵敏度增加，而在较高浓度时检测与标准ELISA相似。与琼脂凝胶沉淀（AGP）试验相比，两种ELISA系统都具有相同的灵敏度提高。