Chertow B S, Blaner W S, Baranetsky N G, Sivitz W I, Cordle M B, Thompson D, Meda P
J Clin Invest. 1987 Jan;79(1):163-9. doi: 10.1172/JCI112778.
We studied the effects of vitamin A deficiency and repletion on rat insulin release and islet cellular retinol binding protein (CRBP) and cellular retinoic acid binding protein (CRABP). Biphasic insulin release from vitamin A-deficient perifused islets was markedly impaired. Release remained impaired with retinoic acid (RA) repletion, 2 micrograms/g diet compared to release from islets of rats repleted with retinol in the form of retinyl palmitate, 4 micrograms/g diet. Release normalized with RA, 8 micrograms/g diet. Vitamin A deficiency did not affect islet insulin content, cell size, number or structure. In vivo, vitamin A-deficient rats had impaired glucose-induced acute insulin release and glucose intolerance, which improved with repletion. Normal islets had greater concentrations of CRBP than CRABP; vitamin A deficiency reduced CRBP but not CRABP levels. We conclude retinol is required for normal insulin secretion. Retinoic acid may substitute for retinol in this function.
我们研究了维生素A缺乏和补充对大鼠胰岛素释放以及胰岛细胞视黄醇结合蛋白（CRBP）和细胞视黄酸结合蛋白（CRABP）的影响。来自维生素A缺乏的灌注胰岛的双相胰岛素释放明显受损。与以棕榈酸视黄酯形式补充视黄醇（4微克/克饮食）的大鼠胰岛释放相比，以2微克/克饮食补充视黄酸（RA）时，释放仍受损。当RA为8微克/克饮食时，释放恢复正常。维生素A缺乏不影响胰岛胰岛素含量、细胞大小、数量或结构。在体内，维生素A缺乏的大鼠葡萄糖诱导的急性胰岛素释放受损且葡萄糖耐量降低，补充后有所改善。正常胰岛中CRBP的浓度高于CRABP；维生素A缺乏降低了CRBP水平，但未降低CRABP水平。我们得出结论，正常胰岛素分泌需要视黄醇。视黄酸在该功能中可能替代视黄醇。
