Blaner W S, Das K, Mertz J R, Das S R, Goodman D S
J Lipid Res. 1986 Oct;27(10):1084-8.
A study was conducted to explore the effects of retinoic acid, fed to retinol-deficient rats, on the tissue distribution and levels of cellular retinol-binding protein (CRBP) and cellular retinoic acid-binding protein (CRABP). Sensitive and specific radioimmunoassays were employed to measure the levels of both CRBP and CRABP. Two groups of six male rats each were fed a purified retinoid-deficient diet supplemented with either: i) retinyl acetate (control group); or ii) retinoic acid (30 mg/kg diet) (retinol deficient-retinoic acid group). The retinoic acid supplementation was begun after 38 days on the retinoid-deficient diet alone, and was continued for 52-54 days. Analysis of the data indicated that only the CRBP level of the proximal epididymis in the retinol-deficient/retinoic acid group differed significantly from (was lower than) the corresponding control level, at the 1% confidence level. CRABP tissue levels did not differ significantly between the two groups. Thus, a moderately large intake of retinoic acid, as the only source of retinoids, had very little effect on the tissue distribution or levels of either its own cellular binding protein (CRABP) or of CRBP. This study provides further information showing that the tissue levels of the cellular retinoid-binding proteins are highly regulated and maintained in rats, even in the presence of marked changes in retinoid nutritional status.
开展了一项研究，以探究给维生素A缺乏的大鼠喂食视黄酸后，其对细胞视黄醇结合蛋白（CRBP）和细胞视黄酸结合蛋白（CRABP）的组织分布及水平的影响。采用灵敏且特异的放射免疫分析法来测定CRBP和CRABP的水平。将两组雄性大鼠，每组六只，喂食纯化的缺乏类维生素A的日粮，并分别补充：i）醋酸视黄酯（对照组）；或ii）视黄酸（30毫克/千克日粮）（维生素A缺乏-视黄酸组）。在仅喂食缺乏类维生素A的日粮38天后开始补充视黄酸，并持续52 - 54天。数据分析表明，仅在1%置信水平下，维生素A缺乏/视黄酸组附睾近端的CRBP水平与相应对照组水平存在显著差异（低于对照组）。两组之间的CRABP组织水平无显著差异。因此，作为类维生素A的唯一来源，适度大量摄入视黄酸对其自身细胞结合蛋白（CRABP）或CRBP的组织分布或水平影响甚微。这项研究提供了进一步的信息，表明即使在类维生素A营养状况发生显著变化的情况下，大鼠体内细胞类维生素A结合蛋白的组织水平仍受到高度调节并维持稳定。
