Lee J W, Roy P
J Gen Virol. 1986 Dec;67 ( Pt 12):2833-7. doi: 10.1099/0022-1317-67-12-2833.
The complete sequence of the double-stranded RNA segment that codes for a non-structural protein (P8) of bluetongue virus serotype 10 has been determined from a cDNA clone inserted into the plasmid pBR322. The segment 10 RNA of the virus (S10 RNA) is deduced to be 822 base pairs long (0.5 X 10(6) daltons) and has an open reading frame in one strand capable of coding for a protein with a calculated size of 25,572 daltons (229 amino acids) and a net charge of +5.5 at neutral pH.
已从插入质粒pBR322的cDNA克隆中确定了编码蓝舌病病毒血清型10非结构蛋白(P8)的双链RNA片段的完整序列。该病毒的第10节段RNA(S10 RNA)推断长度为822个碱基对(0.5×10⁶道尔顿),并且在一条链上有一个开放阅读框,能够编码一种计算大小为25,572道尔顿(229个氨基酸)且在中性pH下净电荷为+5.5的蛋白质。