NagR Is a Pleiotropic and Dual Transcriptional Regulator in .

NagR, belonging to the GntR/HutC family, is a negative regulator that directly represses the and genes, which are involved in GlcNAc transport and utilization in . Our previous work confirmed that the chitinase B gene () of strain Bti75 is also negatively controlled by YvoA, the ortholog of NagR from . In this work, we investigated its regulatory network in Bti75 and found that YvoA is an N-acetylglucosamine utilization regulator primarily involved in GlcNAc catabolism; therefore YvoA is renamed as NagR. The RNA-seq data revealed that 27 genes were upregulated and 14 genes were downregulated in the Δ mutant compared with the wild-type strain. The regulon (exponential phase) was characterized by RNA-seq, bioinformatics software, electrophoretic mobility shift assays, and quantitative real-time reverse transcription PCR. In the Bti75 genome, 19 genes that were directly regulated and 30 genes that were indirectly regulated by NagR were identified. We compiled , and evidence that NagR behaves as a repressor and activator to directly or indirectly influence major biological processes involved in amino sugar metabolism, nucleotide metabolism, fatty acid metabolism, phosphotransferase system, and the Embden-Meyerhof-Parnas pathway.