Azuma Y, Tokunaga T, Takeda Y, Ogawa T, Takagi N
Jpn J Pharmacol. 1986 Oct;42(2):243-51. doi: 10.1254/jjp.42.243.
The role of calcium ions in the activation of guinea pig neutrophil functions was examined by evaluating the effects of calcium antagonists. The data presented here show that calcium antagonists inhibit the activation of guinea pig neutrophil functions elicited by N-formyl-methionyl-leucyl-phenylalanine (FMLP) such as chemotaxis, superoxide anion generation and granule enzyme release in a concentration-dependent manner. The concentrations of calcium antagonists demonstrating the inhibition of neutrophil functions may be somewhat different for each function and higher than that of smooth muscle cells. Calcium ionophore A23187 (A23187) caused superoxide anion generation and granule enzyme release of the neutrophils. A23187 also potentiated the FMLP-induced superoxide anion generation and granule enzyme release of the neutrophils. On the contrary, A23187 neither elicited neutrophil chemotaxis nor affected FMLP-induced neutrophil chemotaxis. These results indicate the possibility that the inhibitory effect of calcium antagonists on the activation of neutrophil functions is probably not simply mediated by inhibition of calcium uptake but also by inhibition of a calcium-dependent intracellular target.
通过评估钙拮抗剂的作用，研究了钙离子在豚鼠中性粒细胞功能激活中的作用。此处呈现的数据表明，钙拮抗剂以浓度依赖的方式抑制由N-甲酰甲硫氨酰亮氨酰苯丙氨酸（FMLP）引发的豚鼠中性粒细胞功能的激活，如趋化性、超氧阴离子生成和颗粒酶释放。显示出对中性粒细胞功能有抑制作用的钙拮抗剂浓度，对于每种功能可能略有不同，且高于平滑肌细胞的浓度。钙离子载体A23187（A23187）可引起中性粒细胞的超氧阴离子生成和颗粒酶释放。A23187还增强了FMLP诱导的中性粒细胞超氧阴离子生成和颗粒酶释放。相反，A23187既不引发中性粒细胞趋化性，也不影响FMLP诱导的中性粒细胞趋化性。这些结果表明，钙拮抗剂对中性粒细胞功能激活的抑制作用可能不仅简单地通过抑制钙摄取介导，还通过抑制钙依赖性细胞内靶点介导。
