Yadav Jay P, Das Suresh C, Dhaka Pankaj, Mukhopadhyay Asish K, Chowdhury Goutam, Naskar Syamal, Malik Satyaveer S
Division of Veterinary Public Health, Indian Veterinary Research Institute, Izatnagar 243 122, India.
Indian Veterinary Research Institute, Eastern Regional Station, 37, Belgachia Road, Kolkata 700 037, India.
Int J Vet Sci Med. 2017 Dec 15;6(1):123-126. doi: 10.1016/j.ijvsm.2017.11.002. eCollection 2018 Jun.
is one of the most important globally recognised gastroenteric pathogen in humans as well as animals. The present study was aimed to know the similarities/divergence among type A isolates of human and animal origin using the pulsed-field gel electrophoresis (PFGE) as a molecular tool. The enterotoxic isolates obtained by screening of human diarrhoeal cases (n = 130), diarrhoeal cases of pig (n = 52) and goat (n = 50), meat samples ., pork (n = 59) and chevon (n = 57) were characterized by standard cultural and biochemical methods followed by PCR Assays. Accordingly, a total of 11 type A characterized isolates (16S rRNA, , and ) recovered from human diarrhoeal cases (n = 3); diarrhoeal cases of pig (n = 2) and goat (n = 2); meat samples . pork (n = 2) and chevon (n = 2) were examined employing PFGE. The observed clustering pattern in PFGE analysis showed the relatedness between isolates from diarrhoeal goat and chevon (90-100%); diarrhoeal pig and pork (65-68%); moreover, isolates from human diarrhoeal cases were exhibiting lineage to cases from goat and pig diarrhoea as well pork and chevon by 62-68% relatedness. The outcome of the present study indicates the probable contamination of this pathogen to the human food chain through faeces from suspected food animals . goat and pig and their improperly cooked meat.
是人类和动物中全球公认的最重要的胃肠病原体之一。本研究旨在使用脉冲场凝胶电泳（PFGE）作为分子工具，了解人类和动物源A型分离株之间的异同。通过筛选人类腹泻病例（n = 130）、猪腹泻病例（n = 52）和山羊腹泻病例（n = 50）、肉类样本（即猪肉（n = 59）和山羊肉（n = 57））获得的产肠毒素分离株，先用标准培养和生化方法进行鉴定，然后进行PCR检测。据此，从人类腹泻病例（n = 3）、猪腹泻病例（n = 2）、山羊腹泻病例（n = 2）、肉类样本（猪肉（n = 2）和山羊肉（n = 2））中总共获得11株经鉴定的A型分离株（16S rRNA等），采用PFGE进行检测。PFGE分析中观察到的聚类模式显示，腹泻山羊和山羊肉分离株之间的相关性为90 - 100%；腹泻猪和猪肉分离株之间的相关性为65 - 68%；此外，人类腹泻病例分离株与山羊和猪腹泻病例以及猪肉和山羊肉分离株的谱系相关性为62 - 68%。本研究结果表明，这种病原体可能通过疑似食用动物（山羊和猪）的粪便及其未煮熟的肉污染人类食物链。
