Neznanov N S, Kolobkov S L, Ivanov V E, Krazhevskaia M V, Gening L V
Mol Gen Mikrobiol Virusol. 1985 Apr(4):26-30.
Provirus from a component of Rauscher leukaemia virus (RLV) has been cloned. The provirus (the size of 5000 b. p.) contains two LTR sequences and shares expressed sequence homology with Mo-MuLV. Restriction analysis and determination of the LTR nucleotide sequence and of the site from 3'-end of proviral genome have shown the cloned provirus to be the SFEV component of RLV. LTR from this cloned provirus contains all sites necessary for transcription: CAAT and TATA sequences, "cap" site and polyadenylation signal. The LTR of the cloned provirus from SFEV component of RLV has been shown to function as a promoter in E. coli cells.
劳舍尔白血病病毒（RLV）一个组分的前病毒已被克隆。该前病毒（大小为5000碱基对）包含两个长末端重复序列（LTR），并与莫洛尼鼠白血病病毒（Mo-MuLV）具有表达序列同源性。限制性分析以及对LTR核苷酸序列和前病毒基因组3'端位点的测定表明，克隆的前病毒是RLV的SFEV组分。来自该克隆前病毒的LTR包含转录所需的所有位点：CAAT和TATA序列、“帽”位点和聚腺苷酸化信号。已证明来自RLV的SFEV组分的克隆前病毒的LTR在大肠杆菌细胞中起启动子的作用。
