[氨基糖苷磷酸转移酶的合成受噬菌体λ的PL启动子控制]
[Synthesis of aminoglycoside phosphotransferase is under control the PL-promoter of phage lambda].
作者信息
Kiselev V I, Gorkun A F, Astashkin E I, Pachkunov D M
出版信息
Mol Gen Mikrobiol Virusol. 1986 May(5):20-3.
PMID:3025708
Abstract
The recombinant plasmid pKP145 PL has been constructed containing the gene for aminoglycosidephosphotransferase (APT). Expression of the APT gene is under the control of lambda bacteriophage PL promoter. Escherichia coli cells harbouring this plasmid synthesize APT in quantity up to 13-15% of the total cellular protein. The technique for isolation of APT from superproducing cells has been elaborated. Preparations of the enzyme devoid of contaminating bacterial proteins have been obtained.
摘要
已构建出含有氨基糖苷磷酸转移酶（APT）基因的重组质粒pKP145 PL。APT基因的表达受λ噬菌体PL启动子的控制。携带该质粒的大肠杆菌细胞能大量合成APT，其产量可达细胞总蛋白的13% - 15%。已完善了从超量生产细胞中分离APT的技术。现已获得不含污染细菌蛋白的酶制剂。
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