Ma Rong, Gong Jianping, Jiang Xiaowei
Department of Hepatobiliary Surgery, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, 400010, China.
Genes Dis. 2017 Jul 18;4(3):149-153. doi: 10.1016/j.gendis.2017.07.003. eCollection 2017 Sep.
With the rapid development of medicine, the studies of genes have become increasingly concerned by more people and being the contend of a great of researches. The next generation sequencing with its own advantages has been widely used in gene research nowadays. It has almost replaced the traditional sequencing methods (such as Sanger sequencing method), and played an important role in a variety of complex disease researches, including breast cancer. The next generation sequencing technology has the advantages of high speed, high throughput and high accuracy. It has been widely used in various cancers (such as prostate cancer, lung cancer, pancreatic cancer, liver cancer, etc.), especially in breast cancer. Moreover, the use of the next generation sequencing technology to make DNA sequence analysis and risk prediction has made a great contribution to the research of breast cancer. We will focus on the application of whole genome sequencing, exon sequencing and targeted gene sequencing in breast cancer gene research.
随着医学的快速发展，基因研究越来越受到人们的关注，并成为众多研究的焦点。新一代测序技术凭借自身优势，如今已在基因研究中广泛应用。它几乎取代了传统测序方法（如桑格测序法），并在包括乳腺癌在内的多种复杂疾病研究中发挥了重要作用。新一代测序技术具有速度快、通量高和准确性高的优点。它已广泛应用于各种癌症（如前列腺癌、肺癌、胰腺癌、肝癌等），尤其是乳腺癌。此外，利用新一代测序技术进行DNA序列分析和风险预测，为乳腺癌的研究做出了巨大贡献。我们将重点关注全基因组测序、外显子测序和靶向基因测序在乳腺癌基因研究中的应用。
