Shibata Hideki
a Department of Applied Biosciences, Graduate School of Bioagricultural Sciences , Nagoya University , Chikusa-ku , Nagoya , Japan.
Biosci Biotechnol Biochem. 2019 Jan;83(1):20-32. doi: 10.1080/09168451.2018.1525274. Epub 2018 Sep 27.
Apoptosis-linked gene 2 (ALG-2) is a Ca-binding protein with five repetitive EF-hand motifs, named penta-EF-hand (PEF) domain. It interacts with various target proteins and functions as a Ca-dependent adaptor in diverse cellular activities. In the cytoplasm, ALG-2 is predominantly localized to a specialized region of the endoplasmic reticulum (ER), called the ER exit site (ERES), through its interaction with Sec31A. Sec31A is an outer coat protein of coat protein complex II (COPII) and is recruited from the cytosol to the ERES to form COPII-coated transport vesicles. I will overview current knowledge of the physiological significance of ALG-2 in regulating ERES localization of Sec31A and the following adaptor functions of ALG-2, including bridging Sec31A and annexin A11 to stabilize Sec31A at the ERES, polymerizing the Trk-fused gene (TFG) product, and linking MAPK1-interacting and spindle stabilizing (MISS)-like (MISSL) and microtubule-associated protein 1B (MAP1B) to promote anterograde transport from the ER.
凋亡相关基因2（ALG-2）是一种具有五个重复EF手基序的钙结合蛋白，称为五聚EF手（PEF）结构域。它与多种靶蛋白相互作用，并在多种细胞活动中作为钙依赖性衔接蛋白发挥作用。在细胞质中，ALG-2主要通过与Sec31A相互作用定位于内质网（ER）的一个特殊区域，即内质网出口位点（ERES）。Sec31A是II型被膜小泡蛋白复合物（COPII）的外被蛋白，从细胞质募集到ERES以形成COPII被膜运输小泡。我将概述目前关于ALG-2在调节Sec31A的ERES定位中的生理意义以及ALG-2的以下衔接功能的知识，包括桥接Sec31A和膜联蛋白A11以在ERES处稳定Sec31A、聚合Trk融合基因（TFG）产物，以及连接丝裂原活化蛋白激酶1相互作用和纺锤体稳定（MISS）样（MISSL）和微管相关蛋白1B（MAP1B）以促进从内质网的顺向运输。
